Anticorps Polyclonal de lapin anti-RPS3

RPS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 15198-1-AP

Synonymes

ribosomal protein S3, OK/SW-cl.26, FLJ27450, FLJ26283, EC:4.2.99.18

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Galerie de données de validation

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  WB analysis of mouse brain using 15198-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15198-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 15198-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15198-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15198-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15198-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 15198-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15198-1-AP

  IP result of anti-RPS3 (IP:15198-1-AP, 4ug; Detection:15198-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 15198-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15198-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15198-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15198-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 15198-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS3 antibody (15198-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules COLO 320, cellules HeLa, cellules NIH/3T3, tissu cérébral de rat, tissu de côlon de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15198-1-AP cible RPS3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: levure
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS3 Protéine recombinante Ag7346
• Nom complet: ribosomal protein S3
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC003137
• Symbole du gène: RPS3
• Identification du gène (NCBI): 6188
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ribosomal protein S3 is a subunit of 40S ribosomal protein, and has two distinct function. RPS3 acts as a ribosome protein that contributes to the domain of the ribosome where translation is initiated, and involves in repair of UV damage as an endonuclease. Besides binding RNA within the 40S ribosomal subunit, RPS3 participates in transcription, and apoptosis.

Publications

Species	Application	Title
yeast	WB	PLoS Genet; Role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes.; Authors - Faza Marius B MB

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