Anticorps Polyclonal de lapin anti-RPS3A

• Phare
• Validé par KD/KO

RPS3A Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 14123-1-AP

Synonymes

40S ribosomal protein S3a, Fte 1, FTE1, Fte-1, MFTL

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Galerie de données de validation

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  WB analysis using 14123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14123-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 14123-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14123-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14123-1-AP

  IP result of anti-RPS3A (IP:14123-1-AP, 4ug; Detection:14123-1-AP 1:6000) with HeLa cells lysate 1600 ug.

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  IHC staining of human colon using 14123-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14123-1-AP (RPS3A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14123-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS3A antibody (14123-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 14123-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human RPS3A (14123-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules A549, cellules HepG2, cellules Jurkat, cellules Raji
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

14123-1-AP cible RPS3A dans les applications de WB, IHC, IF, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, xénope
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS3A Protéine recombinante Ag5273
• Nom complet: ribosomal protein S3A
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC066926
• Symbole du gène: RPS3A
• Identification du gène (NCBI): 6189
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors.

Publications

Species	Application	Title
Xenopus	WB	Neuron; Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function.; Authors - Jean-Michel Cioni
human	WB	Mol Cell Proteomics; Proteomic Profiling Identified Multiple Short-lived Members of the Central Proteome as the Direct Targets of the Addicted oncogenes in Cancer Cells.; Authors - Qi Tong-Gang TG
human	WB	Mol Cell Proteomics; Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.; Authors - Sipeng Li
mouse,human	WB,IHC,IF	Cell Discov; RPS3A positively regulates the mitochondrial function of human periaortic adipose tissue and is associated with coronary artery diseases.; Authors - Yan Tang ; KD Validated
	IF	Cell Rep; On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.; Authors - Christine E. Holt
Xenopus	IF	Cell; Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons.; Authors - Jean-Michel Cioni

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