Anticorps Monoclonal anti-S6 Ribosomal protein

S6 Ribosomal protein Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(19)

CloneNo.

1C3E10

Avis (1)

N° de cat : 66886-1-Ig

Synonymes

RPS6, S6, 1C3E10, 40S ribosomal protein S6, OK/SW-cl.2

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Galerie de données de validation

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  WB analysis using 66886-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66886-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66886-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66886-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 66886-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66886-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 66886-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 66886-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66886-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S6 Ribosomal protein antibody (66886-1-Ig, Clone: 1C3E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 66886-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human S6 Ribosomal protein (66886-1-Ig, Clone:1C3E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7, tissu hépatique de rat
• Résultats positifs en IHC: tissu placentaire humain, tissu d'estomac de rat, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 18 publications below
• IF: See 1 publications below

Informations sur le produit

66886-1-Ig cible S6 Ribosomal protein dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Hamster
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: S6 Ribosomal protein Protéine recombinante Ag6599
• Nom complet: ribosomal protein S6
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29-32 kDa
• Numéro d’acquisition GenBank: BC000524
• Symbole du gène: RPS6
• Identification du gène (NCBI): 6194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Publications

Species	Application	Title
human	WB	Genome Biol; A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells.; Authors - Sooncheol Lee
human	WB	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
human	WB	Cell Mol Life Sci; Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling; Authors - Kunxiang Gong
human	IF	Microbiol Spectr; Structural Basis and Function of the N Terminus of SARS-CoV-2 Nonstructural Protein 1.; Authors - Kaitao Zhao
mouse	WB	Ecotoxicol Environ Saf; Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.; Authors - Yifan Hong
human	WB	Sci Rep; YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma.; Authors - Yuting Zhan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Morgane (Verified Customer) (09-15-2025)

Quite hard to detect on total protein extract, needs at least 50µg protein and longer exposure

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HCT116

S6 Ribosomal protein Antibody Western Blot validation (1/1000 dilution) in HCT116 (Cat no:66886-1-Ig)

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