Anticorps Recombinant de lapin anti-S6 Ribosomal protein

S6 Ribosomal protein Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

6L9

N° de cat : 80208-1-RR

Synonymes

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

---

Galerie de données de validation

• 

  WB analysis using 80208-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80208-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of zebrafish using 80208-1-RR

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 80208-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using S6 Ribosomal protein antibody (80208-1-RR, Clone: 6L9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules RAW 264.7, tissu de poisson-zèbre
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

80208-1-RR cible S6 Ribosomal protein dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

• Réactivité: Humain, poisson-zèbre, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ribosomal protein S6
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000524
• Symbole du gène: RPS6
• Identification du gène (NCBI): 6194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Publications

Species	Application	Title
human	WB	J Biol Chem; TFE3-SLC36A1 axis promotes resistance to glucose starvation in kidney cancer cells; Authors - Suli Lv
mouse	WB	ACS Omega; ROS-responsive MSC-derived Exosome Mimetics Carrying MHY1485 Alleviate Renal Ischemia Reperfusion Injury through Multiple Mechanisms; Authors - Zhiyu Qian
mouse	WB	NPJ Biofilms Microbiomes; Roles of gut microbiome-associated metabolites in pulmonary fibrosis by integrated analysis; Authors - Jie Li
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17A's role in exacerbating radiation-induced lung injury: Autophagy impairment via the PP2A-mTOR pathway; Authors - Liangzhong Liu
human	WB	Free Radic Biol Med; Glucose Deprivation-restoration Induces Labile Iron Overload and Ferroptosis in Renal Tubules Through V-ATPase-mTOR Axis-mediated Ferritinophagy and Iron Release by TPC2; Authors - Zhiyu Qian
human	WB	Biomark Res; Deciphering metabolic heterogeneity in retinoblastoma unravels the role of monocarboxylate transporter 1 in tumor progression; Authors - Junjie Tang

{{ptg:RelatedPrimaryAntibodies}}