Anticorps Polyclonal de lapin anti-RPS7

• Phare
• Validé par KD/KO

RPS7 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 14491-1-AP

Synonymes

40S ribosomal protein S7, DBA8, ribosomal protein S7, RPS7

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 14491-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14491-1-AP (RPS7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14491-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14491-1-AP (RPS7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 14491-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14491-1-AP (RPS7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 14491-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14491-1-AP (RPS7 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HepG2
• Résultats positifs en IHC: tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14491-1-AP cible RPS7 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS7 Protéine recombinante Ag5892
• Nom complet: ribosomal protein S7
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC061901
• Symbole du gène: RPS7
• Identification du gène (NCBI): 6201
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal protein S7(RPS7) belongs to the RPs, which is structural compoent of the ribosome invilved in protein synthesis. It interacts with and inhibit mdm2 mediated p53 degradation function. Also RPS7 has a essential role in early development. RPS7 involves in the maturation of ribosomal RNAs in the large or the small ribosomal subunit production pathway

Publications

Species	Application	Title
mouse	WB	Nat Chem Biol; Identifying the functional contribution of the defatty-acylase activity of SIRT6.; Authors - Xiaoyu Zhang
human	WB	Sci Rep; Stabilization of SIRT7 deacetylase by viral oncoprotein HBx leads to inhibition of growth restrictive RPS7 gene and facilitates cellular transformation.; Authors - Vijaya Pandey
human	WB	Exp Cell Res; Combination of DESI2 and endostatin gene therapy significantly improves antitumor efficacy by accumulating DNA lesions, inducing apoptosis and inhibiting angiogenesis.; Authors - Huaying Yan
human	WB	Cell Death Dis; SMYD2 promotes tumorigenesis and metastasis of lung adenocarcinoma through RPS7.; Authors - Lei Wu
mouse	WB,IHC,IF	Biomed Res Int; Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury; Authors - Hao Zhang ; KD Validated
human	WB	Cell; Human hematopoietic stem cell vulnerability to ferroptosis; Authors - Jiawei Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

H (Verified Customer) (07-09-2020)

This antibody has so many non-specific bands. Considering the fact that ribosomal proteins are so abundant, having these many non-specific bands is not usual for an r-protein antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

RPS7 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:14491-1-AP)