Anticorps Recombinant de lapin anti-RRBP1

RRBP1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230159G10

N° de cat : 82890-3-RR

Synonymes

Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 230159G10, 180 kDa ribosome receptor homolog

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Galerie de données de validation

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  WB analysis using 82890-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82890-3-RR (RRBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 82890-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82890-3-RR (RRBP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IF Staining of HeLa using 82890-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RRBP1 antibody (82890-3-RR, Clone: 230159G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 82890-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RRBP1 Recombinant antibody (82890-3-RR, Clone:230159G10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of U-2 OS using 82890-3-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug RRBP1 Recombinant antibody (82890-3-RR, Clone:230159G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82890-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82890-3-RR against Human RRBP1 were performed. The affinity constant is 3.26 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NCI-H1299
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa, U-2 OS

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82890-3-RR cible RRBP1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RRBP1 Protéine recombinante Ag16648
• Nom complet: ribosome binding protein 1 homolog 180kDa (dog)
• Masse moléculaire calculée: 1410 aa, 152 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: BC128577
• Symbole du gène: RRBP1
• Identification du gène (NCBI): 6238
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis(PMID: 22709790; 8812507; 7686155). RRBP1 has two different isoforms with molecular weights of 150 kDa and 109 kDa.

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