Anticorps Monoclonal anti-RRM1

RRM1 Monoclonal Antibody for FC, IHC, IP, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

4C12E10

N° de cat : 60073-1-Ig

Synonymes

R1, ribonucleotide reductase M1, RIR1, RR1, RRM1

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Galerie de données de validation

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  WB analysis of K-562 using 60073-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60073-1-Ig (RRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 60073-1-Ig

  IP Result of anti-RRM1 (IP:60073-1-Ig, 5ug; Detection:60073-1-Ig 1:1000) with K-562 cells lysate 3200ug.

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  IHC staining of human lung cancer using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60073-1-Ig(RRM1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60073-1-Ig(RRM1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 60073-1-Ig(RRM1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 60073-1-Ig(RRM1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cervical cancer using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60073-1-Ig(RRM1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60073-1-Ig(RRM1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60073-1-Ig(RRM1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60073-1-Ig(RRM1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 60073-1-Ig

  Immunofluorescent analysis of fixed HepG2 cells using 60073-1-Ig (RRM1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

• 

  FC experiment of HepG2 using 60073-1-Ig

  1X10^6 HepG2 cells were stained with .2ug RRM1 antibody (60073-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules K-562
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de carcinome urothélial humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:3000-1:8000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

60073-1-Ig cible RRM1 dans les applications de WB, IP, IHC, IF, FC,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RRM1 Protéine recombinante Ag0789
• Nom complet: ribonucleotide reductase M1
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC006498
• Symbole du gène: RRM1
• Identification du gène (NCBI): 6240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B. RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).

Publications

Species	Application	Title
human	WB,IF	Cell Chem Biol; Clofarabine Commandeers the RNR-α-ZRANB3 Nuclear Signaling Axis.; Authors - Marcus J C Long
human	WB	Oncotarget; The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.; Authors - Lifei Fan
	IHC	World J Surg Oncol; A pilot study: sequential gemcitabine/cisplatin and icotinib as induction therapy for stage IIB to IIIA non-small-cell lung adenocarcinoma.; Authors - Lv Chao C