Anticorps Monoclonal anti-RRM1

• Phare
• Validé par KD/KO

RRM1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

5H6F3

Avis (1)

N° de cat : 60073-2-Ig

Synonymes

R1, ribonucleotide reductase M1, RIR1, RR1, RRM1

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Galerie de données de validation

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  WB analysis of K-562 using 60073-2-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60073-2-Ig (RRM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60073-2-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60073-2-Ig (RRM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 60073-2-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60073-2-Ig (RRM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 60073-2-Ig

  IP Result of anti-RRM1 (IP:60073-2-Ig, 5ug; Detection:60073-2-Ig 1:1000) with K-562 cells lysate 3440ug.

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  IHC staining of human breast cancer using 60073-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60073-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 60073-2-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 60073-2-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60073-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60073-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60073-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60073-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60073-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60073-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60073-2-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RRM1 antibody (60073-2-Ig, Clone: 5H6F3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human breast cancer using 60073-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RRM1 antibody (60073-2-Ig, Clone: 5H6F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 60073-2-Ig

  Immunofluorescent analysis of HeLa cells using 60073-2-Ig (RRM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de cancer du pancréas humain, tissu de cancer du poumon humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa, tissu de cancer du sein humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:3000-1:8000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

60073-2-Ig cible RRM1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RRM1 Protéine recombinante Ag0789
• Nom complet: ribonucleotide reductase M1
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC006498
• Symbole du gène: RRM1
• Identification du gène (NCBI): 6240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).

Publications

Species	Application	Title
human	WB	EMBO Mol Med; PMID: 31950591; Authors - Sean G Rudd
human	IF	Head Neck; ATM, THMS and RRM1 protein expression in nasopharyngeal carcinomas (NPC) treated with curative intent.; Authors - Jenny Jaeeun Ko
human	WB	J Gastrointest Oncol; Abrogation of ARF6 in promoting erastin-induced ferroptosis and mitigating capecitabine resistance in gastric cancer cells.; Authors - Donghua Geng
human	IHC	Oncol Lett; PMID: 28521448; Authors - Toru Aoyama
human	IHC	Zhongguo Fei Ai Za Zhi; [Prognostic Analysis of ERCC1, RRM1 and p53 Expressions in Postoperative Stage I-II Lung Cancer.].; Authors - Huimin Wang
human	WB,IF	Cell Death Discov; Knockdown of RRM1 in tumor cells promotes radio-/chemotherapy induced ferroptosis by regulating p53 ubiquitination and p21-GPX4 signaling axis.; Authors - Yang Gao ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Juan Pablo (Verified Customer) (11-29-2018)

Works great in WB. I treated CGN with shh (1ug/ml) to induce proliferation and I see a nice increase in RRM1 protein. You get a clean band around 90kDa.For Immuno works good at 1:100 dilution. Tested in P7 cerebellum

• Applications: Western Blot, Immunohistochemistry,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cerebellar granule culture from P7 pups.

RRM1 Antibody Western Blot,Immunohistochemistry, validation (1:1000 dilution) in Cerebellar granule culture from P7 pups. (Cat no:60073-2-Ig)