Anticorps Polyclonal de lapin anti-RRN3

RRN3 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, chIP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 25918-1-AP

Synonymes

RNA polymerase I-specific transcription initiation factor RRN3, TIF IA, TIFIA, TIF-IA

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Galerie de données de validation

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  WB analysis of HeLa using 25918-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25918-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25918-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25918-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25918-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25918-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25918-1-AP

  IP result of anti-RRN3 (IP:25918-1-AP, 4ug; Detection:25918-1-AP 1:500) with HeLa cells lysate 3200ug.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules K-562, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

25918-1-AP cible RRN3 dans les applications de WB, IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RRN3 Protéine recombinante Ag23207
• Nom complet: RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC132688
• Symbole du gène: RRN3
• Identification du gène (NCBI): 54700
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	chIP	Biochem Biophys Res Commun; Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression.; Authors - Xiaolei Zhang
	WB	Mol Cell; Control of ribosomal RNA synthesis by hematopoietic transcription factors; Authors - Charles Antony
human	WB	Mol Biol Cell; A role for the kinetochore protein, NUF2, in ribosome biogenesis; Authors - Ty J Brown

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