Anticorps Polyclonal de lapin anti-RSF1

• Phare
• Validé par KD/KO

RSF1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 26011-1-AP

Synonymes

HBV pX associated protein 8, HBXAP, p325, RSF 1, RSF1, XAP8

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Galerie de données de validation

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  WB analysis using 26011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26011-1-AP (RSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26011-1-AP

  WB result of RSF1 antibody (26011-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSF1 transfected HeLa cells.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HEK-293, cellules HeLa, cellules U-251

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

26011-1-AP cible RSF1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RSF1 Protéine recombinante Ag22456
• Nom complet: remodeling and spacing factor 1
• Masse moléculaire calculée: 1441 aa, 164 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC153210
• Symbole du gène: RSF1
• Identification du gène (NCBI): 51773
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RSF1, remodelling and spacing factor 1, is an important interphase centromere protein and is overexpressed in many types of cancers and correlated with poor overall survival. RSF1 has functions mainly in maintaining chromosome stability, facilitating DNA repair, maintaining the protein homeostasis of RSF1 and suppressing the transcription of some oncogenes when RSF1 protein is expressed at an optimal level; however, RSF1 overexpression facilitates drug resistance and cell cycle checkpoint inhibition to prompt cancer proliferation and survival. The RSF1 expression level and gene background are crucial for RSF1 functions, which may explain why RSF1 has different functions in different cancer types. The calculated molecular weight of RSF1 is 163 kDa, but 26011-1-AP can detect a band around 250 kDa, we validated the specificity by knock down. (PMID: 34147108)

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hengbin (Verified Customer) (12-05-2022)

We have generated KO cells and would be happy to test the antibody.

• Applications: Western Blot, ChIP
• Cell Tissue Type: cell or tissues