Anticorps Polyclonal de lapin anti-RSL24D1

RSL24D1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 25190-1-AP

Synonymes

C15orf15, HRP L30 iso, L30, My024, Ribosomal L24 domain-containing protein 1

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Galerie de données de validation

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  WB analysis of HepG2 using 25190-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25190-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 25190-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25190-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 25190-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RSL24D1 antibody (25190-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HL-60, cellules PC-3
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

25190-1-AP cible RSL24D1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RSL24D1 Protéine recombinante Ag18195
• Nom complet: ribosomal L24 domain containing 1
• Masse moléculaire calculée: 163 aa, 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC008409
• Symbole du gène: RSL24D1
• Identification du gène (NCBI): 51187
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RSL24D1 (Ribosomal L24 Domain Containing 1) is a ribosomal protein that is primarily localized in the nucleolus and is involved in the biogenesis of the 60S ribosomal subunit. In mouse embryonic stem cells (ESCs), the expression level of RSL24D1 is high and significantly decreases during cell differentiation. The role of RSL24D1 in ribosome biogenesis includes facilitating proper protein docking in nucleolar and cytoplasmic precursor particles. After the completion of biogenesis, RSL24D1 dissociates from the particles and is thought to be replaced by ribosomal protein L24.

Publications

Species	Application	Title
human	WB,IF	Cell Rep; Labeling of heterochronic ribosomes reveals C1ORF109 and SPATA5 control a late step in human ribosome assembly.; Authors - Chunyang Ni
human	WB	RNA; Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing; Authors - Mason A McCool
human	IF	mBio; Genome-Scale CRISPR Screening Reveals Host Factors Required for Ribosome Formation and Viral Replication; Authors - Maikke B Ohlson
human	WB	Cell; The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrity; Authors - Vidhya Krishnamoorthy
human	WB,IF	Nat Cell Biol; A programmed decline in ribosome levels governs human early neurodevelopment; Authors - Chunyang Ni

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