• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-RSPO3

RSPO3 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 17193-1-AP

Synonymes

CRISTIN1, hPWTSR, hRspo3, Protein with TSP type 1 repeat, Protein with TSP type-1 repeat



Applications testées

Résultats positifs en WBtissu d'intestin grêle de souris, cellules HEK-293 transfectées
Résultats positifs en IPtissu placentaire humain

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

17193-1-AP cible RSPO3 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène RSPO3 Protéine recombinante Ag9899
Nom complet R-spondin 3 homolog (Xenopus laevis)
Masse moléculaire calculée 272 aa, 31 kDa
Poids moléculaire observé 30-40 kDa
Numéro d’acquisition GenBankBC022367
Symbole du gène RSPO3
Identification du gène (NCBI) 84870
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.

Protocole

Product Specific Protocols
WB protocol for RSPO3 antibody 17193-1-APDownload protocol
IP protocol for RSPO3 antibody 17193-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Hepatic stellate cells control liver zonation, size and functions via R-spondin 3

Authors - Atsushi Sugimoto
mouseWB

Nat Commun

R-Spondin chromosome rearrangements drive Wnt-dependent tumour initiation and maintenance in the intestine.

Authors - Teng Han
humanWB

Proc Natl Acad Sci U S A

RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.

Authors - Kendra S Carmon
mouseWB,IF

Cell Death Dis

Endothelial cell-derived RSPO3 activates Gαi1/3-Erk signaling and protects neurons from ischemia/reperfusion injury

Authors - Ting-Tao Liu
  • KD Validated
humanWB

Cardiovasc Res

Role of R-spondin 2 in arterial lymphangiogenesis and atherosclerosis.

Authors - Bhupesh Singla
ratWB

Heliyon

Astragalus polysaccharide improves diabetic ulcers by promoting M2-polarization of macrophages to reduce excessive inflammation via the β-catenin/ NF-κB axis at the late phase of wound-healing

Authors - Zhang Zhen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Gerald (Verified Customer) (07-23-2025)

Worked very well for Western Blot, showing a clear band at the expected molecular weight size, thereby confirming a successful transfection.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/250
RSPO3 Antibody Western Blot validation (1/250 dilution) in (Cat no:17193-1-AP)
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