Anticorps Recombinant de lapin anti-RUFY3

RUFY3 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240519E7

N° de cat : 83601-2-RR

Synonymes

RIPx, Rap2-interacting protein x, Rap2 interacting protein x, Protein RUFY3, KIAA0871

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Galerie de données de validation

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  WB analysis using 83601-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83601-2-RR (RUFY3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 83601-2-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug RUFY3 Recombinant antibody (83601-2-RR, Clone:240519E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83601-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83601-2-RR against Human RUFY3 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules BGC-823, cellules HeLa, cellules SGC-7901, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en FC (Intra): cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83601-2-RR cible RUFY3 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RUFY3 Protéine recombinante Ag35558
• Nom complet: RUN and FYVE domain containing 3
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC051716
• Symbole du gène: RUFY3
• Identification du gène (NCBI): 22902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RUFY3 (RUN and FYVE domain-containing protein 3) was originally described as lacking part of the C-terminal CC2 and FYVE domains. The interaction of RUFY3 with the filamentous actin network through a complex formed together with Rap2 and Fascin 1 (FSCN1) is critical for axonogenesis and growth cone development (PMID: 37463962). RUFY3 also seems to play a crucial role in promoting cancer development (PMID: 34510031).

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