Anticorps Polyclonal de lapin anti-RUNX2

• Phare
• Validé par KD/KO

RUNX2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA

Conjugaison

Non conjugué

Publications(235)

Avis (1)

N° de cat : 20700-1-AP

Synonymes

AML3, AML-3, CBF-alpha-1, Core-binding factor subunit alpha-1, OSF 2

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Galerie de données de validation

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  WB analysis of ROS1728 using 20700-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 20700-1-AP (RUNX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20700-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20700-1-AP (RUNX2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of ROS1728 using 20700-1-AP

  IP result of anti-RUNX2 (IP:20700-1-AP, 4ug; Detection:20700-1-AP 1:1000) with ROS1728 cells lysate 2000 ug.

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  IHC staining of rat kidney using 20700-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20700-1-AP (RUNX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skin using 20700-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 20700-1-AP (RUNX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of ROS1728 using 20700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed ROS1728 cells using RUNX2 antibody (20700-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 20700-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human RUNX2 (20700-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules ROS1728, cellules MCF-7
• Résultats positifs en IP: cellules ROS1728,
• Résultats positifs en IHC: tissu cutané de rat, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules ROS1728,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 200 publications below
• IHC: See 29 publications below
• IF: See 51 publications below
• IP: See 2 publications below
• ChIP: See 3 publications below

Informations sur le produit

20700-1-AP cible RUNX2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: runt-related transcription factor 2
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57-60 kDa
• Numéro d’acquisition GenBank: NM_004348
• Symbole du gène: RUNX2
• Identification du gène (NCBI): 860
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.

Publications

Species	Application	Title
human	WB	Bioact Mater; 3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration; Authors - Lei Zhang
mouse	WB	Small; Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy; Authors - Dan Li
mouse	WB	Redox Biol; Endogenous hydrogen sulfide accelerated trauma-induced heterotopic ossification through the Ca2+/ERK pathway-enhanced aberrant osteogenic activity; Authors - Zhengqiang Yuan
human	WB	Mater Today Bio; Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration.; Authors - Zhi-Hu Zhao
mouse	WB,IF	J Nanobiotechnology; Ultrasound-triggered piezoelectric polyetheretherketone with boosted osteogenesis via regulating Akt/GSK3β/β-catenin pathway; Authors - Yue Li
	WB	ACS Appl Mater Interfaces; Synergistic Effect of Magneto-Mechanical Bioengineered Stem Cells and Magnetic Field to Alleviate Osteoporosis; Authors - Congcong Yu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

YINGJIAN (Verified Customer) (08-15-2025)

high specificity, strong/robust signal, low background, consistent results, reproducible performance, good lot-to-lot consistency

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: heart

RUNX2 Antibody Western Blot validation (1:5000 dilution) in heart (Cat no:20700-1-AP)

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