Anticorps Polyclonal de lapin anti-RUVBL1

• Phare
• Validé par KD/KO

RUVBL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, CHIP, ELISA

Conjugaison

Non conjugué

Publications

44

Avis (1)

N° de cat : 10210-2-AP

Synonymes

49 kDa TBP interacting protein, ECP 54, ECP54, INO80 complex subunit H, INO80H, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, RuvB like 1, RuvB like 1 (E. coli), RUVBL1, RVB1, TAP54 alpha, TIH1, TIP49, TIP49A

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Galerie de données de validation

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  WB analysis using 10210-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10210-2-AP (RUVBL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10210-2-AP

  IP Result of anti-RUVBL1 (IP:10210-2-AP, 3ug; Detection:10210-2-AP 1:1000) with HeLa cells lysate 800ug.

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  IHC staining of mouse liver using 10210-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10210-2-AP (RUVBL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10210-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10210-2-AP (RUVBL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10210-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10210-2-AP (RUVBL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 10210-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10210-2-AP (RUVBL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 10210-2-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RUVBL1 antibody (10210-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HEK-293, cellules HeLa, cellules K-562, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu hépatique de souris, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 14 publications below
• WB: See 39 publications below
• IHC: See 7 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

10210-2-AP cible RUVBL1 dans les applications de WB, IP, IHC, IF, CoIP, CHIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RUVBL1 Protéine recombinante Ag0281
• Nom complet: RuvB-like 1 (E. coli)
• Masse moléculaire calculée: 456 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC002993
• Symbole du gène: RUVBL1
• Identification du gène (NCBI): 8607
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.

Publications

Species	Application	Title
mouse		Nat Immunol; Hierarchical regulation of the resting and activated T cell epigenome by major transcription factor families.; Authors - Yi Zhong
mouse	WB	Mol Cell; Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.; Authors - Sang Gyun Kim
mouse	CHIP	Nat Struct Mol Biol; R loops regulate promoter-proximal chromatin architecture and cellular differentiation.; Authors - Poshen B Chen
human	WB	Sci Adv; Assembly of the asymmetric human γ-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase.; Authors - Fabian Zimmermann ; KD Validated
human	WB	Sci Adv; Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.; Authors - Seung Ho Shin ; KD Validated
human	IP	Genes Dev; SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA.; Authors - Ailone Tichon

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

It worked well in H1 human embryonic stem cell.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human embryonic stem cell

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