Anticorps Polyclonal de lapin anti-RUVBL2

• Phare
• Validé par KD/KO

RUVBL2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (1)

N° de cat : 10195-1-AP

Synonymes

48 kDa TBP interacting protein, CGI 46, ECP 51, ECP51, INO80 complex subunit J, INO80J, Repressing pontin 52, REPTIN, Reptin 52, RuvB like 2, RuvB like 2 (E. coli), RUVBL2, RVB2, TAP54 beta, TIH2, TIP48, TIP49B

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Galerie de données de validation

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  WB analysis using 10195-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10195-1-AP (RUVBL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa cells using 10195-1-AP

  IP result of anti-RUVBL2 (10195-1-AP for IP and Detection).

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  IP experiment of mouse brain using 10195-1-AP

  IP Result of anti-RUVBL2 (IP:10195-1-AP, 4ug; Detection:10195-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human testis using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 10195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RUVBL2 antibody (10195-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules C2C12, cellules Daudi, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa, tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu de gliome humain, tissu de lymphome humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Informations sur le produit

10195-1-AP cible RUVBL2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RUVBL2 Protéine recombinante Ag0253
• Nom complet: RuvB-like 2 (E. coli)
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC000428
• Symbole du gène: RUVBL2
• Identification du gène (NCBI): 10856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RuvB-like 2 (E. coli) (RUVBL2, synonyms: ECP51, TIP48, CGI-46, TIP49B) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that RUVBL2 has both ATPase and DNA helicase activities.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.; Authors - Sang Gyun Kim
human	WB,IF	Sci Adv; Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.; Authors - Seung Ho Shin ; KD Validated
human	WB,IP	Nat Commun; Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.; Authors - Jiawei Zhang
mouse	IP	J Clin Invest; circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.; Authors - Zhenzhen Chen
human	WB	Sci Adv; ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer.; Authors - Franklin C Harwood
mouse	WB	EMBO J; LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.; Authors - Yanying Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

It worked well in H1 human embryonic stem cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human embryonic stem cell