Anticorps Polyclonal de lapin anti-RUVBL2

• Phare
• Validé par KD/KO

RUVBL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(23)

Avis (1)

N° de cat : 10195-1-AP

Synonymes

RUVB2, 48 kDa TATA box-binding protein-interacting protein, 48 kDa TBP-interacting protein, CGI-46, EC:3.6.4.12

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Galerie de données de validation

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  WB analysis using 10195-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10195-1-AP (RUVBL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa cells using 10195-1-AP

  IP result of anti-RUVBL2 (10195-1-AP for IP and Detection).

• 

  IP experiment of mouse brain using 10195-1-AP

  IP result of anti-RUVBL2 (IP:10195-1-AP, 4ug; Detection:10195-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human placenta using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat bladder using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat bladder using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RUVBL2 antibody (10195-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules C2C12, cellules Daudi, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa, tissu cérébral de souris
• Résultats positifs en IHC: tissu vésical de rat, tissu de gliome humain, tissu placentaire humain, tissu vésical de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

10195-1-AP cible RUVBL2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RUVBL2 Protéine recombinante Ag0253
• Nom complet: RuvB-like 2 (E. coli)
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC000428
• Symbole du gène: RUVBL2
• Identification du gène (NCBI): 10856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RuvB-like 2 (E. coli) (RUVBL2, synonyms: ECP51, TIP48, CGI-46, TIP49B) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that RUVBL2 has both ATPase and DNA helicase activities.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.; Authors - Sang Gyun Kim
human	WB,IF	Sci Adv; Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.; Authors - Seung Ho Shin ; KD Validated
human	WB,IP	Nat Commun; Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.; Authors - Jiawei Zhang
mouse	IP	J Clin Invest; circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.; Authors - Zhenzhen Chen
human	WB	Sci Adv; ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer.; Authors - Franklin C Harwood
mouse	WB	EMBO J; LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.; Authors - Yanying Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

It worked well in H1 human embryonic stem cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human embryonic stem cell

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