Anticorps Polyclonal de lapin anti-RWDD1

RWDD1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 17036-1-AP

Synonymes

CGI 24, DFRP2, PTD013, RWD domain containing 1, RWDD1

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Galerie de données de validation

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  WB analysis using 17036-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17036-1-AP (RWDD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 17036-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17036-1-AP (RWDD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17036-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17036-1-AP (RWDD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17036-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17036-1-AP (RWDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 17036-1-AP

  IP Result of anti-RWDD1 (IP:17036-1-AP, 4ug; Detection:17036-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

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  IHC staining of mouse kidney using 17036-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17036-1-AP (RWDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, tissu de thymus de rat, tissu de thymus de souris
• Résultats positifs en IP: tissu de thymus de souris
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

17036-1-AP cible RWDD1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RWDD1 Protéine recombinante Ag10525
• Nom complet: RWD domain containing 1
• Masse moléculaire calculée: 243 aa, 28 kDa
• Poids moléculaire observé: 28-36 kDa
• Numéro d’acquisition GenBank: BC015802
• Symbole du gène: RWDD1
• Identification du gène (NCBI): 51389
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RWDD1(RWD domain-containing protein 1) is also named as DFRP2(DRG family-regulatory protein 2) and belongs to the RWDD1/GIR2 family. RWDD1 is a thymus aging-related protein which contains an RWD domain at its N-terminus and it is expressed in both thymocytes and thymic epithelial cells and located primarily in the cytoplasm. This protein expression in thymus is decreased in aged and oxidatively stressed mice (PMID:18954556). RWDD1 has a calculated molecular mass of 28 kDa, and apparent molecular mass of 32-36 kDa due to its high acidic amino acid composition that results in the slowed migration behavior in electrophoresis (PMID: 30278355, 18954556).

Publications

Species	Application	Title
mouse	WB,IHC	Mol Cell Endocrinol; RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation.; Authors - Gr?tsch Helga H