Anticorps Polyclonal de lapin anti-Rabbit IgG control

Rabbit IgG control Polyclonal Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

n/a

Applications

FC (Intra)

Conjugaison

FITC Fluorescent Dye

Publications(2)

N° de cat : FITC-30000

Synonymes

Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control

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Galerie de données de validation

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  FC experiment of HeLa using FITC-30000

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug FITC Anti-Rabbit IgG control (FITC-30000) (red), or 0.4 ug FITC-conjugated ATF4 Rabbit PolyAb (FITC-10835) (green). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

Résultats positifs en FC (Intra)

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-30000 cible Rabbit IgG control dans les applications de FC (Intra) et montre une réactivité avec des échantillons n/a

• Réactivité: n/a
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: Rabbit IgG control
• Conjugaison: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 498 nm / 526 nm
• Forme: Liquide
• Méthode de purification: N/A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Publications

Species	Application	Title
		Virulence; Exploring the modulatory impact of isosakuranetin on Staphylococcus aureus: Inhibition of sortase A activity and α-haemolysin expression; Authors - Lili Tian
		Nat Commun; Recurrent evolution of adhesive defence systems in amphibians by parallel shifts in gene expression; Authors - Shabnam Zaman

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