Anticorps Monoclonal anti-eqRFP630/eqRFP611
eqRFP630/eqRFP611 Monoclonal Antibody for WB, ELISA
Hôte / Isotype
Mouse / IgG2b
Réactivité testée
Protéine recombinante et plus (3)
Applications
WB, IF, ELISA
Conjugaison
Non conjugué
CloneNo.
1E3C5
N° de cat : 67378-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | ag27669 |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 6 publications below |
| IF | See 1 publications below |
Informations sur le produit
67378-1-Ig cible eqRFP630/eqRFP611 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Protéine recombinante
| Réactivité | Protéine recombinante |
| Réactivité citée | Humain, levure, souris |
| Hôte / Isotype | Mouse / IgG2b |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | eqRFP630/eqRFP611 Protéine recombinante Ag27669 |
| Nom complet | eqRFP630/eqRFP611 |
| Masse moléculaire calculée | 26 kDa |
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. RFP611 and RFP 630 is a basic (constitutively fluorescent) red fluorescent protein derived from Entacmaea quadricolor. This antibody is a mouse (IgG2b) monoclonal antibody raised against RFP (eqFP611), it can also recognize eqFP630. This antibody does not recognize DsRed or mRFP.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for eqRFP630/eqRFP611 antibody 67378-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
bioRxiv Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins | ||
PNAS Nexus Ribosome stalling during c-myc translation presents actionable cancer cell vulnerability | ||
Autophagy The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple | ||
EMBO Rep Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome | ||
Mol Biol Cell Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH April (Verified Customer) (08-28-2025) | Product worked well for detecting RFP in mouse tissues with cells containing RFP.
|
FH Christine (Verified Customer) (08-05-2025) | Tried on total cell lysates of HEK293T transfected with a mCHERRY tagged protein. This RFP antibody was not very good at detecting the mCHERRY tag (mCHERRY antibody from proteintech 68088-1-ig) was better.
|
