Anticorps Polyclonal de lapin anti-S100A14

• Phare
• Validé par KD/KO

S100A14 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

25

N° de cat : 10489-1-AP

Synonymes

Protein S100 A14, S100A14, S100A15, S114

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Galerie de données de validation

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  WB analysis using 10489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10489-1-AP (S100A14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10489-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10489-1-AP (S100A14 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10489-1-AP

  IP result of anti-S100A14(IP:10489-1-AP, 4ug; Detection:10489-1-AP 1:1000) with MCF-7 cells lysate 760 ug.

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  IHC staining of human breast cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10489-1-AP (S100A14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10489-1-AP (S100A14 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10489-1-AP (S100A14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10489-1-AP (S100A14 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10489-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A14 antibody (10489-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, tissu d'estomac de rat
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cutané humain, tissu de cancer du côlon humain, tissu de gliome humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 21 publications below
• IHC: See 17 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

10489-1-AP cible S100A14 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S100A14 Protéine recombinante Ag0757
• Nom complet: S100 calcium binding protein A14
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 10-12 kDa
• Numéro d’acquisition GenBank: BC005019
• Symbole du gène: S100A14
• Identification du gène (NCBI): 57402
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).

Publications

Species	Application	Title
human	WB,IHC	Oncotarget; The role of S100A14 in epithelial ovarian tumors.; Authors - Hanbyoul Cho
human	WB, IHC	Oncogene; S100A14 suppresses metastasis of nasopharyngeal carcinoma by inhibition of NF-kB signaling through degradation of IRAK1.; Authors - Dong-Fang Meng
human	WB, IHC	Int J Cancer; S100A14 is a novel independent prognostic biomarker in the triple-negative breast cancer subtype.; Authors - Sidse Ehmsen
human	WB,IHC,IF	Cell Death Dis; Calcium-binding protein S100A14 induces differentiation and suppresses metastasis in gastric cancer.; Authors - Min Zhu
human	IHC,WB	Am J Cancer Res; A new 7-gene survival score assay for pancreatic cancer patient prognosis prediction.; Authors - Lisi Luo
human	WB,IHC	Eur J Cancer; S100A14 regulates the invasive potential of oral squamous cell carcinoma derived cell-lines in vitro by modulating expression of matrix metalloproteinases, MMP1 and MMP9.; Authors - Sapkota Dipak D ; KD Validated