- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-S100A4
S100A4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Humain, souris et plus (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugaison
Non conjugué
CloneNo.
2G11B4
N° de cat : 66489-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | NK-92 cells, cellules A375, cellules A549, cellules HeLa, cellules HSC-T6, cellules NIH/3T3, cellules PC-3, cellules THP-1 |
| Résultats positifs en IHC | tissu de cancer du poumon humain, cellules K-562, tissu d'amygdalite humain, tissu de cancer du côlon humain, tissu pulmonaire de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF/ICC | cellules HepG2, cellules HeLa |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochimie (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 2 publications below |
| WB | See 11 publications below |
| IHC | See 6 publications below |
| IF | See 16 publications below |
| ELISA | See 1 publications below |
| FC | See 1 publications below |
Informations sur le produit
66489-1-Ig cible S100A4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris
| Réactivité | Humain, souris |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Mouse / IgG2a |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | S100A4 Protéine recombinante Ag9019 |
| Nom complet | S100 calcium binding protein A4 |
| Masse moléculaire calculée | 101 aa, 12 kDa |
| Poids moléculaire observé | 12 kDa |
| Numéro d’acquisition GenBank | BC016300 |
| Symbole du gène | S100A4 |
| Identification du gène (NCBI) | 6275 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for S100A4 antibody 66489-1-Ig | Download protocol |
| IHC protocol for S100A4 antibody 66489-1-Ig | Download protocol |
| IF protocol for S100A4 antibody 66489-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Blood C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse | ||
Adv Sci (Weinh) Meis1 Targets Protein Tyrosine Phosphatase Receptor J in Fibroblast to Retard Chronic Kidney Disease Progression | ||
Cell Death Differ Upregulation of Wilms' Tumor 1 in epicardial cells increases cardiac fibrosis in dystrophic mice. | ||
Acta Pharmacol Sin PU.1/Spi1 exacerbates ischemia-reperfusion induced acute kidney injury via upregulating Gata2 and promoting fibroblast activation | ||
PLoS Pathog S100A4+ macrophages facilitate zika virus invasion and persistence in the seminiferous tubules via interferon-gamma mediation. | ||
Cancers (Basel) Usp22 Overexpression Leads to Aberrant Signal Transduction of Cancer-Related Pathways but Is Not Sufficient to Drive Tumor Formation in Mice |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH CAITLIN (Verified Customer) (03-04-2025) | Worked great, highly specific even when used at higher dilution than recommended
|
FH Caitlin (Verified Customer) (10-15-2024) | Works well, very convenient that the antibody can be used at such high dilutions and still achieve band specificity!
|
FH Shinford (Verified Customer) (10-26-2023) | I have some reservations about the antibody because of its relatively low molecular weight. We attempted to create separate gels with 10% and 12% concentrations and made an effort to run the gels for a limited time to ensure that the entire protein remained within the original gels and membranes. While we were able to detect several bands, their positions lower in the gel led to less than ideal band shapes.
 |
