Anticorps Monoclonal anti-S100A4

• Phare
• Validé par KD/KO

S100A4 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

17

CloneNo.

2G11B4

Avis (1)

N° de cat : 66489-1-Ig

Synonymes

18A2, 42A, Calvasculin, CAPL, Fibroblast-specific protein 1, FSP1, FSP-1, Metastasin, MTS1, P9KA, PEL98, Protein Mts1, Protein S100 A4, S100A4

---

Galerie de données de validation

• 

  WB analysis of HeLa using 66489-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66489-1-Ig

  WB result of S100A4 antibody (66489-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A4 transfected HeLa cells.

• 

  WB analysis of A549 using 66489-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 66489-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66489-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66489-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of K-562 using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded k562 slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66489-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66489-1-Ig (S100A4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66489-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S100A4 antibody (66489-1-Ig, Clone: 2G11B4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules A549, cellules NIH/3T3, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du poumon humain, cellules K-562, tissu d'amygdalite humain, tissu de cancer du côlon humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• FC: See 1 publications below

Informations sur le produit

66489-1-Ig cible S100A4 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: S100A4 Protéine recombinante Ag9019
• Nom complet: S100 calcium binding protein A4
• Masse moléculaire calculée: 101 aa, 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC016300
• Symbole du gène: S100A4
• Identification du gène (NCBI): 6275
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Publications

Species	Application	Title
human	WB	Blood; C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse; Authors - Li-Xue Yang
mouse	IHC	Cell Death Differ; Upregulation of Wilms' Tumor 1 in epicardial cells increases cardiac fibrosis in dystrophic mice.; Authors - Zhenglong Guo
mouse	IF,FC	PLoS Pathog; S100A4+ macrophages facilitate zika virus invasion and persistence in the seminiferous tubules via interferon-gamma mediation.; Authors - Wei Yang
mouse	WB	Cancers (Basel); Usp22 Overexpression Leads to Aberrant Signal Transduction of Cancer-Related Pathways but Is Not Sufficient to Drive Tumor Formation in Mice; Authors - Xianghong Kuang
human	IF	Physiol Genomics; Altered placental ion channel gene expression in preeclamptic high-altitude pregnancies; Authors - Colleen G Julian
rat	WB,IF	J Cell Mol Med; Polydatin inhibits ZEB1-invoked epithelial-mesenchymal transition in fructose-induced liver fibrosis.; Authors - Xiaojuan Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Shinford (Verified Customer) (10-26-2023)

I have some reservations about the antibody because of its relatively low molecular weight. We attempted to create separate gels with 10% and 12% concentrations and made an effort to run the gels for a limited time to ensure that the entire protein remained within the original gels and membranes. While we were able to detect several bands, their positions lower in the gel led to less than ideal band shapes.

• Applications: Western Blot
• Primary Antibody Dilution: 1：500
• Cell Tissue Type: Lung cancer and breast cancer cell lines

S100A4 Antibody Western Blot validation (1：500 dilution) in Lung cancer and breast cancer cell lines (Cat no:66489-1-Ig)