Anticorps Polyclonal de lapin anti-S100A7/Psoriasin

S100A7/Psoriasin Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 13061-1-AP

Synonymes

Protein S100 A7, PSOR1, Psoriasin, S100A7, S100A7/Psoriasin, S100A7c

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Galerie de données de validation

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  WB analysis of MCF-7 using 13061-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 13061-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus cancer using 13061-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 13061-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 13061-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 13061-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using S100A7/Psoriasin antibody (13061-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 2 publications below

Informations sur le produit

13061-1-AP cible S100A7/Psoriasin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S100A7/Psoriasin Protéine recombinante Ag3738
• Nom complet: S100 calcium binding protein A7
• Masse moléculaire calculée: 101 aa, 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC034687
• Symbole du gène: S100A7
• Identification du gène (NCBI): 6278
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A7 (psoriasin), a member of the S100 protein family, is a well-known antimicrobial peptide and a signaling molecule which regulates cellular function and is expressed in many squamous cell carcinomas (SCCs), such as SCC of the skin. It is an EF-hand type calcium binding protein localized in epithelial cells, regulates cell proliferation and differentiation. The protein is overexpressed in hyperproliferative skin diseases, exhibits antimicrobial activities against bacteria and induces immunomodulatory activities.

Publications

Species	Application	Title
human	WB,IHC	J Eur Acad Dermatol Venereol; TOPK promotes the development of psoriasis and worenine alleviates psoriasiform dermatitis by inhibiting TOPK activity; Authors - Hui Lu
human	WB	J Eur Acad Dermatol Venereol; Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.; Authors - T Yuan
human	IHC	BMC Cancer; A novel metabolic subtype with S100A7 high expression represents poor prognosis and immuno-suppressive tumor microenvironment in bladder cancer; Authors - Yun Cai
human	WB	Cell Biol Int; S100A7 promotes the development of human endometriosis by activating NF-κB signaling pathway in endometrial stromal cells.; Authors - Qingqing Sun
human	WB	Oncol Lett; PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV-negative head and neck squamous cell carcinoma.; Authors - Yushen Su
human	WB	Mol Med Rep; TET2 is involved in DNA hydroxymethylation, cell proliferation and inflammatory response in keratinocytes.; Authors - Xinxin Liu