Anticorps Polyclonal de lapin anti-S100A8

• Phare
• Validé par KD/KO

S100A8 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(44)

N° de cat : 15792-1-AP

Synonymes

CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, CFAG

---

Galerie de données de validation

• 

  WB analysis of THP-1 using 15792-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15792-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 15792-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15792-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15792-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 15792-1-AP

  IP result of anti-S100A8 (IP:15792-1-AP, 4ug; Detection:15792-1-AP 1:600) with MCF-7 cells lysate 1400 ug.

• 

  IHC staining of mouse lung using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of THP-1 using 15792-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using S100A8 antibody (15792-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of RAW 264.7 using 15792-1-AP

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human S100A8 (15792-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules THP-1, cellules A549, cellules COLO 320, cellules HL-60, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu de cancer de l'estomac humain, tissu splénique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules THP-1,
• Résultats positifs en FC (Intra): cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 28 publications below
• IHC: See 13 publications below
• IF: See 10 publications below

Informations sur le produit

15792-1-AP cible S100A8 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S100A8 Protéine recombinante Ag8500
• Nom complet: S100 calcium binding protein A8
• Masse moléculaire calculée: 93 aa, 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC005928
• Symbole du gène: S100A8
• Identification du gène (NCBI): 6279
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9(16216873).

Publications

Species	Application	Title
human	IHC	Cell Metab; QDPR deficiency drives immune suppression in pancreatic cancer; Authors - Ji Liu
human	WB	Mol Cell; Hexokinase 1 cellular localization regulates the metabolic fate of glucose.; Authors - Adam De Jesus
human	WB	Cell Rep Med; Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors; Authors - Yiting Wang
mouse	WB,IHC	Nat Commun; S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy.; Authors - Jianjie Li
human	WB	Mol Ther; Bulk and single-cell transcriptome profiling reveal the metabolic heterogeneity in human breast cancers.; Authors - Tian-Jian Yu ; KD Validated
mouse	WB	Phytomedicine; Geniposide via enema alleviates colitis by modulating intestinal flora and bile acid metabolites, inhibiting S100A8/S100A9/NF-κB, and promoting TGR5 inhibition of NLRP3 inflammasome; Authors - Bao-Xin Zheng

{{ptg:RelatedPrimaryAntibodies}}