Anticorps Polyclonal de lapin anti-S100A8

• Phare
• Validé par KD/KO

S100A8 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

26

N° de cat : 15792-1-AP

Synonymes

60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A

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Galerie de données de validation

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  WB analysis of THP-1 using 15792-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 15792-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15792-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15792-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15792-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of RAW 264.7 using 15792-1-AP

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human S100A8 (15792-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules A549, cellules COLO 320
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 20 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• FC: See 1 publications below

Informations sur le produit

15792-1-AP cible S100A8 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S100A8 Protéine recombinante Ag8500
• Nom complet: S100 calcium binding protein A8
• Masse moléculaire calculée: 93 aa, 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC005928
• Symbole du gène: S100A8
• Identification du gène (NCBI): 6279
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9(16216873).

Publications

Species	Application	Title
human	WB	Mol Cell; Hexokinase 1 cellular localization regulates the metabolic fate of glucose.; Authors - Adam De Jesus
mouse	WB,IHC	Nat Commun; S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy.; Authors - Jianjie Li
human	WB	Mol Ther; Bulk and single-cell transcriptome profiling reveal the metabolic heterogeneity in human breast cancers.; Authors - Tian-Jian Yu ; KD Validated
rat	WB	Acta Physiol (Oxf); Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.; Authors - Li Zhang
mouse	WB	Mol Cell Proteomics; Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury; Authors - Yi-Han Lin
human	WB	Int J Mol Sci; Human Isogenic Cell Line Models for Neutrophils and Myeloid-Derived Suppressor Cells.; Authors - Yuting Zhang

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