Anticorps Recombinant de lapin anti-S100A8

S100A8 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

242970C4

N° de cat : 98411-3-RR

Synonymes

CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, CFAG

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Galerie de données de validation

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  FC experiment of human peripheral blood lymphocytes using 98411-3-RR

  1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.25 ug Anti-Human S100A8 Rabbit RecAb (98411-3-RR, Clone: 242970C4),or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human peripheral blood lymphocytes using 98411-3-RR

  1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.25 ug Anti-Human S100A8 Rabbit RecAb (98411-3-RR, Clone: 242970C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): lymphocytes de sang périphérique humain

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98411-3-RR cible S100A8 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: S100A8 Protéine recombinante Eg3752
• Nom complet: S100 calcium binding protein A8
• Masse moléculaire calculée: 93 aa, 11 kDa
• Numéro d’acquisition GenBank: BC005928
• Symbole du gène: S100A8
• Identification du gène (NCBI): 6279
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.

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