Anticorps Recombinant de lapin anti-S100A8

S100A8 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

250113D8

N° de cat : CL594-98467-2

Synonymes

Caga, Calgranulin-A, Chemotactic cytokine CP-10, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using CL594-98467-2

  1x10^6 untransfected or S100A8-transfected HEK-293T cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse S100A8 Rabbit RecAb (CL594-98467-2, Clone: 250113D8). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Transfected HEK-293T using CL594-98467-2

  1x10^6 untransfected or S100A8-transfected HEK-293T cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse S100A8 Rabbit RecAb (CL594-98467-2, Clone: 250113D8) (red), or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): Transfected HEK-293T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-98467-2 cible S100A8 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: S100A8 Protéine recombinante Eg2959
• Nom complet: S100 calcium binding protein A8 (calgranulin A)
• Masse moléculaire calculée: 10 kDa
• Numéro d’acquisition GenBank: NM_013650.2
• Symbole du gène: S100a8
• Identification du gène (NCBI): 20201
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.

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