Anticorps Polyclonal de lapin anti-S100A9

• Phare
• Validé par KD/KO

S100A9 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 14226-1-AP

Synonymes

60B8AG, CAGB, Calgranulin B, Calprotectin L1H subunit, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP 14, MRP14, NIF, P14, Protein S100 A9, S100A9

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Galerie de données de validation

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  WB analysis of mouse spleen using 14226-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14226-1-AP

  WB result of S100A9 antibody (14226-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A9 transfected MCF-7 cells.

• 

  WB analysis of human heart using 14226-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14226-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 14226-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 14226-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 14226-1-AP (S100A9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14226-1-AP (S100A9 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14226-1-AP (S100A9 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14226-1-AP (S100A9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human tonsillitis using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14226-1-AP (S100A9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14226-1-AP (S100A9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14226-1-AP (S100A9 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules MCF-7, sang humain, Sanguin humain, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

14226-1-AP cible S100A9 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S100A9 Protéine recombinante Ag5465
• Nom complet: S100 calcium binding protein A9
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 14 kDa, 28 kDa
• Numéro d’acquisition GenBank: BC047681
• Symbole du gène: S100A9
• Identification du gène (NCBI): 6280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

Publications

Species	Application	Title
human,mouse	IHC,IF	Int J Cancer; S100 calcium-binding protein A9 from tumor-associated macrophage enhances cancer stem cell-like properties of hepatocellular carcinoma.; Authors - Ran Wei
human,mouse	WB,IHC	Cell Biosci; TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4α signaling.; Authors - Qiang Liu ; KD Validated
rat	IF	Int J Med Sci; Single-cell RNA Sequencing Uncovered the Involvement of an Endothelial Subset in Neutrophil Recruitment in Chemically Induced Rat Pulmonary Inflammation.; Authors - Hong Huang
human	WB,IF	J Oncol; Downregulation of S100A9 Reverses Cisplatin-Resistance and Inhibits Proliferation and Migration in Hypopharyngeal Carcinoma; Authors - Shiyu Zeng ; KD Validated
human	IHC	Histol Histopathol; Histopathological assessment of calcification and inflammation of calcific aortic valves from patients with and without diabetes mellitus.; Authors - Josephin Mosch
human,rat	IF	Burns Trauma; S100 calcium-binding protein A9 promotes skin regeneration through toll-like receptor 4 during tissue expansion; Authors - Yu Zhang

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