Anticorps Polyclonal de lapin anti-S100P

S100P Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 11803-1-AP

Synonymes

MIG9, Protein S100 E, Protein S100 P, S100 calcium binding protein P, S100E, S100P

---

Galerie de données de validation

• 

  WB analysis of HeLa using 11803-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11803-1-AP (S100P antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11803-1-AP (S100P antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11803-1-AP (S100P antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 11803-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using S100P antibody (11803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 11803-1-AP

  Immunofluorescent analysis of HepG2 cells, using S100P antibody 11803-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HepG2 using 11803-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug S100P antibody (11803-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 5 publications below

Informations sur le produit

11803-1-AP cible S100P dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S100P Protéine recombinante Ag2379
• Nom complet: S100 calcium binding protein P
• Masse moléculaire calculée: 95 aa, 11 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC006819
• Symbole du gène: S100P
• Identification du gène (NCBI): 6286
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.

Publications

Species	Application	Title
human	WB, IHC	Am J Cancer Res; High expression of S100P is associated with unfavorable prognosis and tumor progression in patients with epithelial ovarian cancer.; Authors - Xiangyu Wang
human	IHC	Proteomics Clin Appl; Identification of distinctive protein expression patterns in colorectal adenoma.; Authors - F Lam Francis F
human	IHC	Diagn Pathol; Basal cell adenocarcinoma of the salivary gland: a morphological and immunohistochemical comparison with basal cell adenoma with and without capsular invasion.; Authors - Jung Min Jung MJ
human	WB	Exp Ther Med; Proof-of-concept study investigating the role of S100P-RAGE in nasopharyngeal carcinoma.; Authors - Chengyu Wang
human	WB	Technol Cancer Res Treat; LHPP Inhibits the Viability, Migration, and Proliferation of PDAC Cells and Significantly Affects the Expression of SDC1 and S100p; Authors - Zhaozhi Xia
human	IHC	Int J Mol Sci; Expression-Based Diagnosis, Treatment Selection, and Drug Development for Breast Cancer; Authors - Qing Ye