Anticorps Polyclonal de lapin anti-S1PR2

• Phare
• Validé par KD/KO

S1PR2 Polyclonal Antibody for WB, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Conjugaison

Non conjugué

Publications(57)

Avis (1)

N° de cat : 21180-1-AP

Synonymes

AGR16, EDG 5, EDG5, Endothelial differentiation G-protein coupled receptor 5, Gpcr13

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Galerie de données de validation

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  WB analysis using 21180-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21180-1-AP (S1PR2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 21180-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using S1PR2 antibody (21180-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 21180-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed frozen OCT-embedded mouse brain tissue using 21180-1-AP (S1PR2 antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HuH-7, cellules MCF-7, cellules MDA-MB-231
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF-Fro: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 48 publications below
• IHC: See 10 publications below
• IF: See 13 publications below
• IP: See 2 publications below

Informations sur le produit

21180-1-AP cible S1PR2 dans les applications de WB, IHC, IF-P, IF-Fro, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S1PR2 Protéine recombinante Ag15470
• Nom complet: sphingosine-1-phosphate receptor 2
• Masse moléculaire calculée: 353 aa, 39 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC069598
• Symbole du gène: S1PR2
• Identification du gène (NCBI): 9294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S1PR2 (sphingosine-1-phosphate receptor 2), also known as S1P2 or EDG5, is one of five G-protein-coupled receptors for sphingosine-1-phosphate (S1P), a biologically active metabolic product of sphingolipid (PMID: 18787560). S1P and its receptors have important regulatory functions in normal physiology and disease processes, particularly involving the immune, central nervous, and cardiovascular systems (PMID: 21339489). S1PR2 plays a role in acute vascular inflammation (PMID: 23723450). S1PR2 signaling is implicated in STAT3 activation (PMID: 22606352).

Publications

Species	Application	Title
mouse	WB	Gastroenterology; Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells; Authors - Decai Wang
mouse	WB,IP,IHC	Brain; Gamma subunit of complement component 8 is a neuroinflammation inhibitor.; Authors - Jong-Heon Kim
mouse,human	IHC,IF	Nat Commun; Critical role of sphingosine-1-phosphate receptor-2 in the disruption of cerebrovascular integrity in experimental stroke.; Authors - Gab Seok Kim ; KO Validated
human	WB	EMBO Mol Med; Therapeutic development of group B Streptococcus meningitis by targeting a host cell signaling network involving EGFR.; Authors - Ningyu Zhu ; KO Validated
mouse	WB	Theranostics; Endothelial S1pr2 regulates post-ischemic angiogenesis via AKT/eNOS signaling pathway.; Authors - Caixia Zhou ; KO Validated
human	IHC	Front Immunol; Altered sphingolipid pathway in SARS-CoV-2 infected human lung tissue; Authors - Rabisa J Khan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Edma (Verified Customer) (03-03-2025)

The antibody quite nice in western blot, but I have to test it in other cell types

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549 cells

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