Anticorps Polyclonal de lapin anti-S1PR5/EDG8

S1PR5/EDG8 Polyclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 13874-1-AP

Synonymes

Edg 8, EDG8, S1P receptor 5, S1P receptor Edg 8, S1P5, S1PR5, SPPR 1, SPPR 2

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Galerie de données de validation

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  WB analysis of mouse brain using 13874-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13874-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13874-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13874-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13874-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  FC experiment of SH-SY5Y using 13874-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug S1PR5/EDG8 antibody (13874-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

13874-1-AP cible S1PR5/EDG8 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S1PR5/EDG8 Protéine recombinante Ag4928
• Nom complet: sphingosine-1-phosphate receptor 5
• Masse moléculaire calculée: 398 aa, 42 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC034703
• Symbole du gène: S1PR5
• Identification du gène (NCBI): 53637
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sphingosine 1-phosphate (S1P) is a biologically active lysophospholipid that elicits diverse physiological effect on most types of cells and tissues via five G protein-coupled receptors, S1PR1 to S1PR5. S1PRs differ in their tissue expression and cell lineage: S1PR1-3 are expressed throughout the immune, central nervous system (CNS), and cardiovascular system; S1PR4 is primarily expressed in lymphoid tissue; and S1PR5 is a predominant receptor in CNS white matter, spleen and natural killer cells (PMID: 21707490). S1PR5 (also known as EDG8) has been involved in the trafficking of NK cells and monocytes (PMID: 21911833; 23519784).

Publications

Species	Application	Title
human	WB	PLoS One; Sphingosine 1-phosphate (S1P) reduces hepatocyte growth factor-induced migration of hepatocellular carcinoma cells via S1P receptor 2.; Authors - Rie Matsushima-Nishiwaki
mouse	WB	Drug Des Devel Ther; Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence.; Authors - Dianna Liu

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