Anticorps Polyclonal de lapin anti-SACM1L

• Phare
• Validé par KD/KO

SACM1L Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 13033-1-AP

Synonymes

KIAA0851, SAC1, SACM1L

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Galerie de données de validation

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  WB analysis of human kidney using 13033-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13033-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13033-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13033-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13033-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 13033-1-AP

  IP Result of anti-SACM1L (IP:13033-1-AP, 4ug; Detection:13033-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human brain using 13033-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13033-1-AP (SACM1L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 13033-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13033-1-AP (SACM1L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 13033-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13033-1-AP (SACM1L Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13033-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13033-1-AP (SACM1L Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 13033-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 13033-1-AP (SACM1L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal humain, cellules A549, tissu cérébral humain, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 15 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Informations sur le produit

13033-1-AP cible SACM1L dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SACM1L Protéine recombinante Ag3682
• Nom complet: SAC1 suppressor of actin mutations 1-like (yeast)
• Masse moléculaire calculée: 587 aa, 67 kDa
• Poids moléculaire observé: 60-67 kDa
• Numéro d’acquisition GenBank: BC016559
• Symbole du gène: SACM1L
• Identification du gène (NCBI): 22908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SACM1L(Suppressor of actin mutations 1-like protein) is also named KIAA0851. It is a phosphoinositide phosphatase that hydrolyzes PtdIns3P and PtdIns4P and has low activity towards PtdIns(3,5)P2.

Publications

Species	Application	Title
human,mouse	WB	Cell Res; Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity; Authors - Ying Gao
	IP	Cell Host Microbe; Global mapping of Salmonella enterica-host protein-protein interactions during infection.; Authors - Philipp Walch
mouse	WB	Neuron; An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.; Authors - Jochen Schwenk
human	WB	Autophagy; PtdIns4P restriction by hydrolase SAC1 decides specific fusion of autophagosomes with lysosomes.; Authors - Hongjun Zhang ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.; Authors - Lei Shi
human	WB,IF	J Cell Biol; Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking.; Authors - Maria Casas