Anticorps Polyclonal de lapin anti-SAE1

SAE1 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10229-1-AP

Synonymes

AOS1, FLJ3091, HSPC140, SAE1, SUA1, UBLE1A

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Galerie de données de validation

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  WB analysis of A431 using 10229-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10229-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 10229-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10229-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10229-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10229-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10229-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 10229-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 10229-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 10229-1-AP

  Immunofluorescent analysis of HeLa cells using 10229-1-AP (SAE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules COLO 320, cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

10229-1-AP cible SAE1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SAE1 Protéine recombinante Ag0275
• Nom complet: SUMO1 activating enzyme subunit 1
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC000344
• Symbole du gène: SAE1
• Identification du gène (NCBI): 10055
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. It had an apparent molecular mass of 40 kD by SDS-PAGE. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).

Publications

Species	Application	Title
mouse	WB	Curr Biol; Pumilio 1 suppresses multiple activators of p53 to safeguard spermatogenesis.; Authors - Dong Chen
human	IHC	Open Med (Wars); Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation.; Authors - Yang Liu
human	WB	Nat Commun; SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation; Authors - Lingjie Yan
human	IHC,WB	Curr Issues Mol Biol; SUMO-Activating Enzyme Subunit 1 Is Associated with Poor Prognosis, Tumor Progression, and Radio-Resistance in Colorectal Cancer; Authors - Yueh-Jung Wu
human	WB	Elife; TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.; Authors - Xiancai Ma