Anticorps Polyclonal de lapin anti-SAMD4A

• Phare
• Validé par KD/KO

SAMD4A Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 17387-1-AP

Synonymes

hSmaug1, KIAA1053, Protein Smaug homolog 1, SAMD4, SAMD4A, Smaug, Smaug 1, Smaug1, SMG, SMGA

---

Galerie de données de validation

• 

  WB analysis of mouse skeletal muscle using 17387-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17387-1-AP (SAMD4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 17387-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17387-1-AP (SAMD4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 17387-1-AP

  IP result of anti-SAMD4A (IP:17387-1-AP, 4ug; Detection:17387-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug.

• 

  IHC staining of human spleen using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cardiaque de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu splénique humain, tissu ovarien humain, tissu pulmonaire humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

17387-1-AP cible SAMD4A dans les applications de WB, IHC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SAMD4A Protéine recombinante Ag11273
• Nom complet: sterile alpha motif domain containing 4A
• Masse moléculaire calculée: 529 aa, 59 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC021726
• Symbole du gène: SAMD4A
• Identification du gène (NCBI): 23034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Mol Immunol; SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA.; Authors - Yuze Wang ; KO Validated
mouse	WB	J Neuroinflammation; Smad7 in the hippocampus contributes to memory impairment in aged mice after anesthesia and surgery; Authors - Changliang Liu
human	IHC,WB	Tissue Cell; SAMD4A serves as a negative prognostic marker for gastric cancer patients; Authors - Deqin Li ; KD Validated
human	WB	Elife; YAP/TAZ enhances P-body formation to promote tumorigenesis; Authors - Xia Shen
human	WB	Cell Host Microbe; MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids; Authors - George D Moschonas
human	WB	Sci Rep; GNG2 inhibits brain metastases from colorectal cancer via PI3K/AKT/mTOR signaling pathway; Authors - Chenhua Luo

{{ptg:RelatedPrimaryAntibodies}}