Anticorps Polyclonal de lapin anti-SAMHD1

• Phare
• Validé par KD/KO

SAMHD1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

65

N° de cat : 12586-1-AP

Synonymes

DCIP, HDDC1, Monocyte protein 5, MOP 5, MOP5, SAM domain and HD domain 1, SAMHD1, SBBI88

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Galerie de données de validation

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  WB analysis using 12586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12586-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12586-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12586-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12586-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12586-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12586-1-AP

  IP Result of anti-SAMHD1 (IP:12586-1-AP, 3ug; Detection:12586-1-AP 1:300) with K-562 cells lysate 2400ug.

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  IHC staining of human lung cancer using 12586-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12586-1-AP (SAMHD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12586-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12586-1-AP (SAMHD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12586-1-AP (SAMHD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 12586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12586-1-AP (SAMHD1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SAMHD1 antibody (12586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of THP-1 using 12586-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.2 ug Anti-Human SAMHD1 (12586-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), or 0.2 ug Control Antibody (black). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

• 

  FC experiment of K-562 using 12586-1-AP

  1X10^6 K-562 cells were stained with 0.2ug SAMHD1 antibody (12586-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules K-562, cellules L02, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral humain
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules THP-1, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 14 publications below
• WB: See 42 publications below
• IHC: See 9 publications below
• IF: See 10 publications below
• IP: See 3 publications below
• FC: See 11 publications below

Informations sur le produit

12586-1-AP cible SAMHD1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SAMHD1 Protéine recombinante Ag3287
• Nom complet: SAM domain and HD domain 1
• Masse moléculaire calculée: 626 aa, 72 kDa
• Poids moléculaire observé: 64-72 kDa
• Numéro d’acquisition GenBank: BC036450
• Symbole du gène: SAMHD1
• Identification du gène (NCBI): 25939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.

Publications

Species	Application	Title
human	IF	Nat Genet; Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response.; Authors - Rice Gillian I GI
human	IF	Nat Microbiol; Human cytomegalovirus overcomes SAMHD1 restriction in macrophages via pUL97.; Authors - Ramona Businger
human	WB,IHC	Nat Med; PMID: 27991919; Authors - Constanze Schneider
human	WB,IHC	Nat Med; PMID: 28067901; Authors - Nikolas Herold
human	WB, IHC, IF	Nat Med; SAMHD1 restricts HIV-1 infection in resting CD4(+) T cells.; Authors - Baldauf Hanna-Mari HM ; KD Validated
monkey	WB	Immunity; Tissue Myeloid Cells in SIV-Infected Primates Acquire Viral DNA through Phagocytosis of Infected T Cells.; Authors - Nina Calantone

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