Anticorps Polyclonal de lapin anti-SAP130

SAP130 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 12130-1-AP

Synonymes

SAP130

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Galerie de données de validation

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  WB analysis of HEK-293 using 12130-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12130-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12130-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12130-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12130-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12130-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 12130-1-AP

  IP Result of anti-SAP130 (IP:12130-1-AP, 4ug; Detection:12130-1-AP 1:600) with HepG2 cells lysate 1000ug.

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  IHC staining of human pancreas cancer using 12130-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12130-1-AP (SAP130 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12130-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12130-1-AP (SAP130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

12130-1-AP cible SAP130 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SAP130 Protéine recombinante Ag2774
• Nom complet: Sin3A-associated protein, 130kDa
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC017453
• Symbole du gène: SAP130
• Identification du gène (NCBI): 79595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SF3b is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3b contains the spliceosomal proteins SAP 49, SAP 130, SAP 145, and SAP 155. SAP 130, SAP 145, and SAP 155 are present in a protein complex and associate with one another. While SAP 155 and SAP 130 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.