Anticorps Polyclonal de lapin anti-SAR1B

SAR1B Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 22292-1-AP

Synonymes

ANDD, CMRD, GTBPB, GTP binding protein B, GTP binding protein SAR1b, SAR1 homolog B (S. cerevisiae), SAR1B, SARA2, SARB

---

Galerie de données de validation

• 

  WB analysis of mouse liver using 22292-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 22292-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22292-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 22292-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22292-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 22292-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu cérébral de souris, tissu de muscle squelettique de souris, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu de muscle squelettique humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

22292-1-AP cible SAR1B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SAR1 homolog B (S. cerevisiae)
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 25-27 kDa
• Numéro d’acquisition GenBank: NM_016103
• Symbole du gène: SAR1B
• Identification du gène (NCBI): 51128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex. The antibody is specific to SAR1B.

Publications

Species	Application	Title
human	WB,IP	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen ; KO Validated
mouse	WB,IF	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
mouse,human	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
rat	WB	J Biol Chem; The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.; Authors - Lee G D Fryer
mouse	WB	J Biol Chem; Small intestine but not liver lysophosphatidylcholine acyltransferase 3 (Lpcat3) deficiency has a dominant effect on plasma lipid metabolism.; Authors - Inamul Kabir