Anticorps Polyclonal de lapin anti-SASS6

SASS6 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 21377-1-AP

Synonymes

HsSAS 6, HsSAS-6, SAS 6, SAS6, Spindle assembly abnormal protein 6 homolog

---

Galerie de données de validation

• 

  WB analysis of HeLa using 21377-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21377-1-AP (SASS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21377-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21377-1-AP (SASS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21377-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21377-1-AP (SASS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21377-1-AP

  IP result of anti-SASS6 (IP:21377-1-AP, 4ug; Detection:21377-1-AP 1:1000) with HeLa cells lysate 600ug.

• 

  IP experiment of HeLa cells using 21377-1-AP

  IP result of anti-SAS6 (21377-1-AP for IP and Detection) with HeLa cells.

• 

  IHC staining of mouse testis using 21377-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21377-1-AP (SASS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 21377-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21377-1-AP (SASS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21377-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 21377-1-AP (SASS6 Antibody) at dilution of 1:50.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu testiculaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

21377-1-AP cible SASS6 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SASS6 Protéine recombinante Ag15917
• Nom complet: spindle assembly 6 homolog (C. elegans)
• Masse moléculaire calculée: 657 aa, 74 kDa
• Poids moléculaire observé: 70-74 kDa
• Numéro d’acquisition GenBank: BC101026
• Symbole du gène: SASS6
• Identification du gène (NCBI): 163786
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Spindle assembly abnormal protein 6 homolog (SASS6) is a centrosomal protein that, together with PLK4, CEP135, CENPJ (also known as CPAP) and STIL, is involved in regulating the number of centrosomes in human cells.

Publications

Species	Application	Title
human	WB	Curr Biol; SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.; Authors - Hindol Gupta
human	WB,IHC	Oncol Rep; Knockdown of SASS6 reduces growth of MDA‑MB‑231 triple‑negative breast cancer cells through arrest of the cell cycle at the G2/M phase.; Authors - Lili Du
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
human	IF	EBioMedicine; Bi-allelic variants in CEP295 cause Seckel-like syndrome presenting with primary microcephaly, developmental delay, intellectual disability, short stature, craniofacial and digital abnormalities; Authors - Niu Li
human	WB	Clin Exp Med; SASS6 promotes tumor proliferation and is associated with TP53 and immune infiltration in lung adenocarcinoma; Authors - Zihao Li

{{ptg:RelatedPrimaryAntibodies}}