Anticorps Polyclonal de lapin anti-SATB1

• Phare
• Validé par KD/KO

SATB1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 15400-1-AP

Synonymes

Special AT-rich sequence-binding protein 1, DNA-binding protein SATB1

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Galerie de données de validation

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  WB analysis of human brain using 15400-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15400-1-AP (SATB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15400-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15400-1-AP (SATB1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15400-1-AP

  IP result of anti-SATB1 (IP:15400-1-AP, 4ug; Detection:15400-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human oesophagus cancer using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris, tissu splénique de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, tissu cérébral de rat, tissu de carcinome urothélial humain, tissu de thymus de rat, tissu de thymus de souris, tissu splénique de rat, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

15400-1-AP cible SATB1 dans les applications de WB, IHC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SATB1 Protéine recombinante Ag7615
• Nom complet: SATB homeobox 1
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 86-100 kDa
• Numéro d’acquisition GenBank: BC001744
• Symbole du gène: SATB1
• Identification du gène (NCBI): 6304
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Epigenetic modifications and dynamic changes in chromatin organization by organizer proteins have recently been shown to play an instrumental role in regulating cancer-promoting genes. Special AT-rich binding protein (SATB1) is a unique type of global regulator that integrates higher-order chromatin organization -withregulation of gene expression. [PMID:23076250,22998183,23121661] SATB1 is a T cell-enriched transcription factor and a chromatin organizer essential for controlling genes that participate in T-cell development and activation. It regulates gene expression by periodically anchoring matrix attachment regions to the nuclear matrix and directly recruiting chromatin-modifying factors. Depending on its posttranslational modifications, SATB1 activates or represses multiple genes.Its expression is regulated by interleukin-4 (IL4) during T helper-2(Th2) cell differentiation[PMID: 20522714]. The calculated molecular weight of SATB1 is 86 kDa, but modified SATB1 is about 100 kDa (PMID: 22879953).

Publications

Species	Application	Title
		Dev Cell; Folding Keratin Gene Clusters during Skin Regional Specification.; Authors - Ya-Chen Liang
human	WB,IP	Cell Rep; SATB1 regulates 3D genome architecture in T cells by constraining chromatin interactions surrounding CTCF-binding sites; Authors - Bao Wang ; KO Validated
mouse,rat	WB	Pharmacol Res; Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.; Authors - Jiale Cai
mouse	WB	Mol Neurobiol; SATB1/SLC7A11/HO-1 Axis Ameliorates Ferroptosis in Neuron Cells After Ischemic Stroke by Danhong Injection; Authors - Sikai Zhan
human	WB	Eur Rev Med Pharmacol Sci; Effect of STAT3 mediated epigenetic regulation in pre-eclampsia: an analysis of trial data.; Authors - X-L Zhang
human	WB	Int J Med Sci; SATB1 Knockdown Inhibits Proliferation and Invasion and Decreases Chemoradiation Resistance in Nasopharyngeal Carcinoma Cells by Reversing EMT and Suppressing MMP-9.; Authors - Dongni Zhou ; KD Validated

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