Anticorps Polyclonal de lapin anti-SBNO1

SBNO1 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 25359-1-AP

Synonymes

Monocyte protein 3, MOP 3, MOP3, SBNO1, Sno

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Galerie de données de validation

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  WB analysis using 25359-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25359-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25359-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 25359-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25359-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25359-1-AP

  IP result of anti-SBNO1(IP:25359-1-AP, 4ug; Detection:25359-1-AP 1:3000) with Jurkat cells lysate 1400 ug.

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  IF Staining of HeLa using 25359-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 25359-1-AP (SBNO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules Raji, tissu cérébral de souris
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

25359-1-AP cible SBNO1 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SBNO1 Protéine recombinante Ag19005
• Nom complet: strawberry notch homolog 1 (Drosophila)
• Masse moléculaire calculée: 1393 aa, 154 kDa
• Poids moléculaire observé: 145-154 kDa
• Numéro d’acquisition GenBank: BC133704
• Symbole du gène: SBNO1
• Identification du gène (NCBI): 55206
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SBNO1, also named as Monocyte protein 3 or MOP3, is a 1393 amino acid protein, which belongs to the SBNO family. SBNO1 play an important role in development of the vertebrate central nervous system. SBNO1 has four isoforms with MW 154 kDa and 80 kDa.

Publications

Species	Application	Title
mouse	WB,IF	Asian J Androl; Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice.; Authors - Cong Shen