Anticorps Polyclonal de lapin anti-SCFD1

• Phare
• Validé par KD/KO

SCFD1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 12569-1-AP

Synonymes

C14orf163, KIAA0917, RA410, SCFD1, SLY1, SLY1 homolog, Sly1p, STXBP1L2

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Galerie de données de validation

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  WB analysis of A549 using 12569-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12569-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12569-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis using 12569-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12569-1-AP

  IP Result of anti-SCFD1 (IP:12569-1-AP, 3ug; Detection:12569-1-AP 1:500) with HeLa cells lysate 2150ug.

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  IHC staining of human endometrial cancer using 12569-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 12569-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 12569-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12569-1-AP (SCFD1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 12569-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12569-1-AP (SCFD1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293T, cellules NIH/3T3, tissu hépatique humain, tissu rénal humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

12569-1-AP cible SCFD1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Bactérie, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCFD1 Protéine recombinante Ag3234
• Nom complet: sec1 family domain containing 1
• Masse moléculaire calculée: 642 aa, 72 kDa
• Poids moléculaire observé: 66-72 kDa
• Numéro d’acquisition GenBank: BC017734
• Symbole du gène: SCFD1
• Identification du gène (NCBI): 23256
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCFD1, also named as C14orf163, KIAA0917, STXBP1L2, Sly1p and SLY1, belongs to the STXBP/unc-18/SEC1 family. It is a component of the syntaxin-5 SNARE complex which regulates the early secretory pathway of eukaryotic cells at the level of endoplasmic reticulum (ER) to Golgi transport.(PMID:21242315) SCFD1 plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. It is involved in vesicular transport between the endoplasmic reticulum and the Golgi.

Publications

Species	Application	Title
human	WB	EMBO J; Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing.; Authors - Laufman Orly O
human	WB,IP	Autophagy; Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion.; Authors - Hong Huang ; KD Validated
human	WB	J Cell Sci; Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.; Authors - Renna Maurizio M ; KD Validated
Bacteria	WB	Microb Pathog; Formononetin alleviates Streptococcus suis infection by targeting suilysin.; Authors - Guizhen Wang
	WB	J Appl Microbiol; Silibinin attenuates Streptococcus suis serotype 2 virulence by targeting suilysin.; Authors - Xue Shen