Anticorps Polyclonal de lapin anti-Uteroglobin/CC10

Uteroglobin/CC10 Polyclonal Antibody for FC (Intra), IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC (Intra), FC, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 10490-1-AP

Synonymes

CC10, CC16, CCPBP, CCSP, SCGB1A1, UGB, UP 1, UP1, Urinary protein 1, Urine protein 1, Uteroglobin, Uteroglobin/CC10

---

Galerie de données de validation

• 

  IHC staining of human lung cancer using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of A549 using 10490-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Uteroglobin/CC10 antibody (10490-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse lung using 10490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Uteroglobin/CC10 antibody (10490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A549 using 10490-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human Uteroglobin/CC10 (10490-1-AP) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en IHC: tissu pulmonaire de souris, tissu de cancer du poumon humain, tissu pulmonaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu pulmonaire de souris, cellules A549
• Résultats positifs en cytométrie: cellules A549

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• FC: See 3 publications below

Informations sur le produit

10490-1-AP cible Uteroglobin/CC10 dans les applications de WB, IP, IHC, IF, FC (Intra), FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Uteroglobin/CC10 Protéine recombinante Ag0758
• Nom complet: secretoglobin, family 1A, member 1 (uteroglobin)
• Masse moléculaire calculée: 10 kDa
• Numéro d’acquisition GenBank: BC004481
• Symbole du gène: SCGB1A1
• Identification du gène (NCBI): 7356
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.

Publications

Species	Application	Title
human	IHC	Nature; SARS-CoV-2 Omicron variant replication in human bronchus and lung ex vivo.; Authors - Kenrie P Y Hui
human	IHC	Lancet Respir Med; Tropism, replication competence, and innate immune responses of the coronavirus SARS-CoV-2 in human respiratory tract and conjunctiva: an analysis in ex-vivo and in-vitro cultures.; Authors - Kenrie P Y Hui
human	IHC	Theranostics; A SCID mouse-human lung xenograft model of SARS-CoV-2 infection.; Authors - Wenkun Fu
human	IF	PLoS Pathog; Lung adenocarcinoma originates from retrovirus infection of proliferating type 2 pneumocytes during pulmonary post-natal development or tissue repair.; Authors - Murgia Claudio C
mouse	IHC	J Pathol; Overexpression of the SARS-CoV-2 receptor ACE2 is induced by cigarette smoke in bronchial and alveolar epithelia.; Authors - Aibin Liu
human	WB	Antioxidants (Basel); Empagliflozin-Enhanced Antioxidant Defense Attenuates Lipotoxicity and Protects Hepatocytes by Promoting FoxO3a- and Nrf2-Mediated Nuclear Translocation via the CAMKK2/AMPK Pathway.; Authors - Yangyang Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tessa (Verified Customer) (02-03-2019)

Used in 20pcw human fetal lung and got nice staining. Looks very specific with low background. Found that it did not require antigen retrieval in cryosections. In fact it turned out better in sections where 10mM Na Citrate buffer at pH6 was not used.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Fetal Lung

Produits associés

CD14 Polyclonal antibody

17000-1-AP

CD14 Monoclonal antibody

60253-1-Ig

Surfactant protein D Polyclonal antibody

11839-1-AP

PTGDS Polyclonal antibody

10754-2-AP

PTGDS Polyclonal antibody

10754-2-AP

LMBR1L Polyclonal antibody

12154-1-AP

Osteopontin Polyclonal antibody

22952-1-AP

MMP9 (N-terminal) Polyclonal antibody

10375-2-AP

Fibronectin Polyclonal antibody

15613-1-AP