Anticorps Polyclonal de lapin anti-SCRIB

SCRIB Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 27083-1-AP

Synonymes

CRIB1, hScrib, KIAA0147, LAP4, Protein LAP4

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Galerie de données de validation

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  WB analysis using 27083-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27083-1-AP (SCRIB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27083-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27083-1-AP (SCRIB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3,
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

27083-1-AP cible SCRIB dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCRIB Protéine recombinante Ag25795
• Nom complet: scribbled homolog (Drosophila)
• Masse moléculaire calculée: 1630 aa, 175 kDa
• Poids moléculaire observé: 260 kDa
• Numéro d’acquisition GenBank: BC036905
• Symbole du gène: SCRIB
• Identification du gène (NCBI): 23513
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCRIB encodes a large, 260-kDa cytoplasmic scaffolding protein that comprises a large leucine-rich repeat (LRR) region and 4 PDZ domains that regulate protein-protein interactions. In mammals, cell polarity is established and maintained by at least 3 protein modules (Scrib, Crumbs, and Par). The apical (Crumbs and Par) and basolateral (Scrib) modules function in a mutually antagonistic relationship to regulate various polarization processes such as apical-basal polarity, planar cell polarity, asymmetric cell division and migration. The loss of SCRIB in malignant tumors suggest that it has potential to be a tumor suppressor. Alteration of polarity through deletion, downregulation, overexpression, and mislocalization can induce structural and functional alteration of cells that might be related to tumorigenesis. Mislocalization of SCRIB is involved in epithelial-to-mesenchymal transition (EMT) by inducing loss of E-cadherin. Deregulated SCRIB expression induces tumorigenesis in breast and liver. (PMID: 28460446, PMID: 32564009)

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; MiR-9-5p Inhibits the MMP+-Induced Neuron Apoptosis through Regulating SCRIB/β-Catenin Signaling in Parkinson's Disease.; Authors - Zhenyong Xiao
mouse	WB,IF	Life Sci Alliance; SLMAP3 is essential for neurulation through mechanisms involving cytoskeletal elements, ABP, and PCP; Authors - Taha Rehmani

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (06-20-2024)

Excellent for detection of Scribble by WB in endothelial cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

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