Anticorps Polyclonal de lapin anti-EMAP II

• Phare
• Validé par KD/KO

EMAP II Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 11091-1-AP

Synonymes

AIMP1, EMAP 2, EMAP II, EMAP2, EMAPII, p43, SCYE1

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Galerie de données de validation

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  WB analysis of A549 using 11091-1-AP

  WB result of EMAP II antibody (11091-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMAP II transfected A549 cells.

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  WB analysis using 11091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11091-1-AP (EMAP II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SGC-7901 using 11091-1-AP

  IP result of anti-EMAP II (IP:11091-1-AP, 4ug; Detection:11091-1-AP 1:500) with SGC-7901 cells lysate 2200 ug.

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  IHC staining of human cervical cancer using 11091-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11091-1-AP (EMAP II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 11091-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11091-1-AP (EMAP II antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11091-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EMAP II antibody (11091-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules SGC-7901, cellules SH-SY5Y, cellules U-251, tissu cérébral de souris
• Résultats positifs en IP: cellules SGC-7901,
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11091-1-AP cible EMAP II dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EMAP II Protéine recombinante Ag1560
• Nom complet: small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa and 68 kDa
• Numéro d’acquisition GenBank: BC014051
• Symbole du gène: SCYE1
• Identification du gène (NCBI): 9255
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kDa precursor can be cleaved to produce an active 20-kDa product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).

Publications

Species	Application	Title
human	WB	Cancer Lett; Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.; Authors - Xintian Chen
human	WB	J Biol Chem; Structural control of caspase-generated glutamyl-tRNA synthetase by appended noncatalytic WHEP domains.; Authors - Dalia Halawani
human	WB	Proteomics; Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins in Laryngeal Squamous Cell Carcinoma.; Authors - Wei Gao
rat	IF	Int J Mol Med; Changes in the expression of endothelial monocyte‑activating polypeptide II in the rat hippocampus following status epilepticus.; Authors - Chun Li
human	IHC,WB	Cancer Commun (Lond); AIMP1 promotes multiple myeloma malignancy through interacting with ANP32A to mediate histone H3 acetylation; Authors - Rongfang Wei
human	WB	Proc Natl Acad Sci U S A; Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex; Authors - Krishnendu Khan ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Krishnendu (Verified Customer) (02-27-2020)

Very strong antibody and works nicely for both WB and IP.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000 for WB and 1:100 for IP
• Cell Tissue Type: HEK293T