Anticorps Polyclonal de lapin anti-SCYL2

SCYL2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12325-1-AP

Synonymes

CVAK104, KIAA1360, SCY1 like 2 (S. cerevisiae), SCY1 like protein 2, SCYL2

---

Galerie de données de validation

• 

  WB analysis using 12325-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12325-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 12325-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 12325-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12325-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12325-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12325-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 12325-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12325-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 12325-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 12325-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules Jurkat, cellules NIH/3T3, tissu utérin de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

12325-1-AP cible SCYL2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCYL2 Protéine recombinante Ag2988
• Nom complet: SCY1-like 2 (S. cerevisiae)
• Masse moléculaire calculée: 929 aa, 104 kDa
• Poids moléculaire observé: 104 kDa
• Numéro d’acquisition GenBank: BC012387
• Symbole du gène: SCYL2
• Identification du gène (NCBI): 55681
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	J Neurosci; SCYL2 Protects CA3 Pyramidal Neurons from Excitotoxicity during Functional Maturation of the Mouse Hippocampus.; Authors - Sebastien Gingras
human	WB,IF	J Cell Biochem; Phosphorylated LASS2 inhibits prostate carcinogenesis via negative regulation of Wnt/β-catenin signaling.; Authors - Kuangen Zhang