Anticorps Polyclonal de lapin anti-CD138/Syndecan-1

• Phare
• Validé par KD/KO

CD138/Syndecan-1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(42)

N° de cat : 10593-1-AP

Synonymes

CD138, SDC, SDC1, SYND1, syndecan

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Galerie de données de validation

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  WB analysis using 10593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10593-1-AP

  WB result of CD138/Syndecan-1 antibody (10593-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD138/Syndecan-1 transfected HeLa cells.

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  IHC staining of human tonsillitis using 10593-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10593-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10593-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules Raji
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 13 publications below
• IF: See 11 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

10593-1-AP cible CD138/Syndecan-1 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD138/Syndecan-1 Protéine recombinante Ag0823
• Nom complet: syndecan 1
• Masse moléculaire calculée: 32.5 kDa
• Poids moléculaire observé: 60-100 kDa, 130 kDa
• Numéro d’acquisition GenBank: BC008765
• Symbole du gène: CD138
• Identification du gène (NCBI): 6382
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.

Publications

Species	Application	Title
human	IF	Cell; Tumor-reactive antibodies evolve from non-binding and autoreactive precursors.; Authors - Roei D Mazor
human	WB,IP	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
mouse	IHC	Leukemia; miR-21 antagonism abrogates Th17 tumor promoting functions in multiple myeloma.; Authors - Marco Rossi
mouse	IF	Theranostics; Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production.; Authors - Xin Chen
		Mol Neurodegener; Amelioration of amyloid-β-induced deficits by DcR3 in an Alzheimer's disease model.; Authors - Yi-Ling Liu
human	WB	Cell Mol Life Sci; The association of telomere maintenance and TERT expression with susceptibility to human papillomavirus infection in cervical epithelium.; Authors - Li-Na Wang

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