Anticorps Polyclonal de lapin anti-Syntenin-1

• Phare
• Validé par KD/KO

Syntenin-1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 22399-1-AP

Synonymes

MDA 9, MDA9, Scaffold protein Pbp1, SDCBP, ST1, SYCL, Syndecan binding protein 1, Syntenin-1, TACIP18

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Galerie de données de validation

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  WB analysis using 22399-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22399-1-AP (Syntenin-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22399-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22399-1-AP (Syntenin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 22399-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 22399-1-AP (Syntenin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22399-1-AP

  IP Result of anti-Syntenin-1 (IP:22399-1-AP, 3ug; Detection:22399-1-AP 1:1000) with HeLa cells lysate 2500ug.

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  IHC staining of human liver cancer using 22399-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 22399-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 22399-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 22399-1-AP (Syntenin-1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 22399-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 22399-1-AP (Syntenin-1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 22399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Syntenin-1 antibody (22399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 22399-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Syntenin-1 antibody (22399-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules MCF-7, tissu placentaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 2 publications below

Informations sur le produit

22399-1-AP cible Syntenin-1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Syntenin-1 Protéine recombinante Ag18078
• Nom complet: syndecan binding protein (syntenin)
• Masse moléculaire calculée: 298 aa, 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC113674
• Symbole du gène: SDCBP
• Identification du gène (NCBI): 6386
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Syntenin-1, also known as SDCBP (syndecan binding protein) and MDA-9 (melanoma differentiation-associated protein 9), was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. Syntenin-1 is a PDZ-domain-containing molecule that has many interaction partners, and regulates transmembrane-receptor trafficking, cell adhesion, tumor-cell metastasis and neuronal-synapse function. Syntenin-1 is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus.

Publications

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei ; KO Validated
human,mouse	WB	Cell Res; Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity; Authors - Ying Gao
human	WB	Cell Mol Life Sci; Exosomes from TNF-α preconditioned human umbilical cord mesenchymal stromal cells inhibit the autophagy of acinar cells of severe acute pancreatitis via shuttling bioactive metabolites; Authors - Zhilong Ma
human	WB	Cell Oncol (Dordr); EV-miRome-wide profiling uncovers miR-320c for detecting metastatic colorectal cancer and monitoring the therapeutic response.; Authors - Yi-Tung Chen
human	WB	Cell Oncol (Dordr); Circular RNA circNFATC3 acts as a miR-9-5p sponge to promote cervical cancer development by upregulating SDC2.; Authors - Ningye Ma
human	WB,IHC	Front Oncol; Syntenin Regulated by miR-216b Promotes Cancer Progression in Pancreatic Cancer.; Authors - Fuqiang Zu ; KD Validated