Anticorps Polyclonal de lapin anti-SDHB

• Phare
• Validé par KD/KO

SDHB Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC (Intra), FC, ELISA

Conjugaison

Non conjugué

Publications

39

Avis (2)

N° de cat : 10620-1-AP

Synonymes

IP, PGL4, SDH, SDH1, SDHB, SDHIP

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Galerie de données de validation

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  WB analysis using 10620-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10620-1-AP

  These five antibodies, ATP5A1 (14676-1-AP, 1:8000), UQCRC2 (14742-1-AP, 1:4000), SDHB (10620-1-AP, 1:8000), MTCO2 (55070-1-AP, 1:1000), NDUFB8 (14794-1-AP, 1:5000), can be assembled OXPHOS kit to detect the relative levels of the 5 OXPHOS complexes in mitochondrial preparations.

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  WB analysis of HepG2 using 10620-1-AP

  WB result of SDHB antibody (10620-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SDHB transfected HepG2 cells.

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  WB analysis of mouse liver using 10620-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10620-1-AP

  IP result of anti-SDHB(IP:10620-1-AP, 4ug; Detection:10620-1-AP 1:5000) with HEK-293 cells lysate 1360 ug.

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  IHC staining of human heart using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse kidney using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse kidney using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 10620-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10620-1-AP (SDHB antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10620-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SDHB (10620-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HEK-293, cellules HeLa, cellules HepG2, tissu cardiaque de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu cardiaque humain, tissu de carcinome à cellules rénales humain, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 34 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

10620-1-AP cible SDHB dans les applications de WB, IP, IHC, IF, FC (Intra), FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SDHB Protéine recombinante Ag0968
• Nom complet: succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: BC007840
• Symbole du gène: SDHB
• Identification du gène (NCBI): 6390
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).

Publications

Species	Application	Title
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang ; KD Validated
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
mouse	WB	Diabetes; CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis; Authors - Meng Ding
mouse	WB	Free Radic Biol Med; Aristolochic acid I exposure triggers ovarian dysfunction by activating NLRP3 inflammasome and affecting mitochondrial homeostasis; Authors - Ming-Xin Sun
human	IF	Oxid Med Cell Longev; 5-Lipoxygenase Inhibition Protects Retinal Pigment Epithelium from Sodium Iodate-Induced Ferroptosis and Prevents Retinal Degeneration.; Authors - Jong-Jer Lee
mouse	WB	Environ Pollut; Accumulation of polystyrene microplastics induces liver fibrosis by activating cGAS/STING pathway.; Authors - Rong Shen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate SDHB works quite well, both with ECL detection and fluorescent secondary antibodies it gives a strong band

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: K562 and KCL22

FH

Lana (Verified Customer) (07-15-2020)

SDS-PAGE: 20 ug/ul RIPA protein lysate. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:5000.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.Lines of WB image: 1 – protein ladder, 2 - whole cell lysate, 3 – mitochondrial fraction lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse embryonic stem cells

SDHB Antibody Western Blot validation (1:5000 dilution) in Mouse embryonic stem cells (Cat no:10620-1-AP)