Anticorps Polyclonal de lapin anti-SDPR

• Phare
• Validé par KD/KO

SDPR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 12339-1-AP

Synonymes

Caveolae-associated protein 2, Cavin 2, Cavin-2, Phosphatidylserine-binding protein, PS p68

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Galerie de données de validation

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  WB analysis of A549 using 12339-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 12339-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12339-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12339-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 12339-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12339-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12339-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12339-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12339-1-AP

  IP result of anti-SDPR (IP:12339-1-AP, 4ug; Detection:12339-1-AP 1:1000) with mouse heart tissue lysate 8000ug.

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  IHC staining of human renal cell carcinoma using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12339-1-AP (SDPR antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast hyperplasia using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast hyperplasia using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 12339-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SDPR antibody (12339-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules COLO 320, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de souris, tissu placentaire de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu cardiaque humain, tissu d'amygdalite humain, tissu d'hyperplasie mammaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12339-1-AP cible SDPR dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SDPR Protéine recombinante Ag2990
• Nom complet: serum deprivation response (phosphatidylserine binding protein)
• Masse moléculaire calculée: 425 aa, 47 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC016475
• Symbole du gène: SDPR
• Identification du gène (NCBI): 8436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Serum deprivation-response protein(SDRP), a substrate of protein kinase C, is a member of a group of proteins that bind to phosphatidylserine in a clacium-dependent manner. SDPR may participate in the generation or stabilization of membrane curvature. SDPR is highly expressed in heart and lung, and low in brain,kidney, liver, pancreas, placenta and skeletal muscle. This is a rabbit polyclonal anti-SDPR antibody raised against C-terminus of human SDPR. SDRP of molecular masses 115 kDa and 100 kDa were found in neutrophils, monocytes, lymphocytes, platelets and erythrocytes. In platelets, however, the principal SDRP is a cytosolic protein of molecular mass 68 kDa. (PMID: 2390065 )

Publications

Species	Application	Title
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao ; KD Validated
mouse		Cardiovasc Res; Loss of Cavin-2 destabilizes PTEN and enhances Akt signaling pathway in cardiomyocytes; Authors - Naoki Maruyama
human	IF	Dev Cell; Cellular heterogeneity of human fallopian tubes in normal and hydrosalpinx disease states identified using scRNA-seq.; Authors - Nicole D Ulrich
mouse	WB	Haematologica; Dynamin 2 is required for GPVI signaling and platelet hemostatic function in mice.; Authors - Nathan Eaton
mouse	WB,IP,IF	Mol Metab; Requirement of Cavin-2 for the expression and stability of IRβ in adequate adipocyte differentiation.; Authors - Yusuke Higuchi
rat	IF	J Cereb Blood Flow Metab; P-glycoprotein traffics from the nucleus to the plasma membrane in rat brain endothelium during inflammatory pain.; Authors - Margaret E Tome

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