Anticorps Polyclonal de lapin anti-SEC14L1

• Phare
• Validé par KD/KO

SEC14L1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 25541-1-AP

Synonymes

SEC14-like protein 1, SEC14L

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Galerie de données de validation

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  WB analysis using 25541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25541-1-AP (SEC14L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 25541-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 25541-1-AP (SEC14L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 25541-1-AP

  IP result of anti-SEC14L1 (IP:25541-1-AP, 4ug; Detection:25541-1-AP 1:500) with HEK-293 cells lysate 1480 ug.

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  IP experiment of HEK-293 using 25541-1-AP

  IP result of anti-SEC14L1 (IP:25541-1-AP, 4ug; Detection:25541-1-AP 1:300) with HEK-293 cells lysate 2600ug.

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  IF Staining of A431 using 25541-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SEC14L1 antibody (25541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Y79
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

25541-1-AP cible SEC14L1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEC14L1 Protéine recombinante Ag22233
• Nom complet: SEC14-like 1 (S. cerevisiae)
• Masse moléculaire calculée: 719 aa, 82 kDa
• Poids moléculaire observé: 81 kDa, 77 kDa
• Numéro d’acquisition GenBank: BC142979
• Symbole du gène: SEC14L1
• Identification du gène (NCBI): 6397
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SEC14L1 (SEC14-like protein 1) is a member of SEC14 family in mammals with the potential to control local phospholipid content in membranes. SEC14L1 may act as a phospholipid transfer protein. It has also been reported that SEC14L1 functions as a novel negative regulator of RIG-I-mediated antiviral signaling and is involved in innate immunity (PMID: 17092608; 23843640). SEC14L1 has three isoforms with the molecular mass of 81.2kDa, 81.8kDa and 77.1kDa.

Publications

Species	Application	Title
human	WB	Elife; The ORP9-ORP11 dimer promotes sphingomyelin synthesis; Authors - Birol Cabukusta ; KO Validated
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi

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