Anticorps Polyclonal de lapin anti-SEC24A

• Phare
• Validé par KD/KO

SEC24A Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 15958-1-AP

Synonymes

SEC24 related protein A, SEC24A

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Galerie de données de validation

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  WB analysis of HeLa using 15958-1-AP

  WB result of SEC24A antibody (15958-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEC24A transfected HeLa cells.

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  WB analysis using 15958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat thymus using 15958-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15958-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15958-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 15958-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 15958-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15958-1-AP (SEC24A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 15958-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15958-1-AP (SEC24A antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15958-1-AP

  Immunofluorescent analysis of Hela cells, using SEC24A antibody 15958-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules COS-7, cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3, tissu de muscle squelettique de souris, tissu de thymus de rat, tissu de thymus de souris, tissu hépatique humain
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

15958-1-AP cible SEC24A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEC24A Protéine recombinante Ag8723
• Nom complet: SEC24 family, member A (S. cerevisiae)
• Masse moléculaire calculée: 613 aa, 66 kDa
• Poids moléculaire observé: 66 kDa, 120 kDa
• Numéro d’acquisition GenBank: BC019341
• Symbole du gène: SEC24A
• Identification du gène (NCBI): 10802
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). Two isoforms of 120 kDa and 66 kDa produced by alternative splicing have been described.

Publications

Species	Application	Title
mouse	WB	Nature; The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1.; Authors - Jinbo Han
human	IF	Cell Metab; The hypercholesterolemia-risk gene SORT1 facilitates PCSK9 secretion.; Authors - Camilla Gustafsen
mouse	WB	J Clin Invest; The COPII cargo adapter SEC24C is essential for neuronal homeostasis.; Authors - Bo Wang
mouse	WB,IF	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human,mouse	WB,IF	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
human	WB,IF,IP	J Immunother Cancer; THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.; Authors - Chushu Li ; KD Validated