Anticorps Polyclonal de lapin anti-SEC31A

• Phare
• Validé par KD/KO

SEC31A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (3)

N° de cat : 17913-1-AP

Synonymes

SEC31L1, SEC31 related protein A, SEC31 like protein 1, KIAA0905, ABP130

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Galerie de données de validation

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  WB analysis using 17913-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17913-1-AP (SEC31A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17913-1-AP

  IP result of anti-SEC31A (IP:17913-1-AP, 4ug; Detection:17913-1-AP 1:5000) with HeLa cells lysate 1320 ug.

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  IHC staining of mouse brain using 17913-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17913-1-AP (SEC31A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17913-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17913-1-AP (SEC31A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 17913-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17913-1-AP (SEC31A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17913-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SEC31A antibody (17913-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 17913-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SEC31A antibody (17913-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 17913-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17913-1-AP (SEC31A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Informations sur le produit

17913-1-AP cible SEC31A dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEC31A Protéine recombinante Ag12314
• Nom complet: SEC31 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 1220 aa, 133 kDa
• Poids moléculaire observé: 118-134 kDa
• Numéro d’acquisition GenBank: BC084583
• Symbole du gène: SEC31A
• Identification du gène (NCBI): 22872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologues of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.

Publications

Species	Application	Title
human	IP,WB	Nature; CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.; Authors - Lingyun Long ; KO Validated
mouse	IF	Cell; Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy.; Authors - Moran Dvela-Levitt
mouse	IF	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang
human	IF	Dev Cell; SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.; Authors - Rui Yan
human,mouse	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (09-01-2020)

This antibody labels a strong band around the expected molecular weight.

• Applications: Western Blot

FH

RICHA (Verified Customer) (09-29-2019)

Clean, excellent and specific

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein from mouse tail tendon

SEC31A Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:17913-1-AP)

FH

Rui (Verified Customer) (09-28-2019)

Labels intracellular vesicles with clear contrast. Cell monolayer fixed by 0.3% glutaraldehyde and 3% paraformaldehyde. Permeabilization: 0.1% saponin and 3% BSA in PBS. Primary antibody overnight at 4C.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Cos-7

SEC31A Antibody Immunofluorescence, validation (1:300 dilution) in Cos-7 (Cat no:17913-1-AP)

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